Anti-CD34 抗体 [QBEND / 10] (Biotin) (ab30375)

製品の概要

  • 製品名
    Anti-CD34 antibody [QBEND / 10] (Biotin)
    CD34 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [QBEND / 10] to CD34 (Biotin)
  • 標識
    Biotin
  • アプリケーション
    適用あり: Flow Cyt, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Human, Cynomolgus monkey, Rhesus monkey
    非交差種: Rat, Sheep, Cow, Dog
  • 免疫原

    Human endothelial cell membrane vesicles.

  • 特記事項
    Each test is 10ul therefore the antibody volume supplied is 500ul.


    This antibody binds to the Class II epitope of CD34

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 10µl for 106 cells. in 100µl.



ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • 組織特異性
    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • 配列類似性
    Belongs to the CD34 family.
  • 発生段階
    On early hematopoietic progenitor cells.
  • 翻訳後修飾
    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

画像

  • ab30375 staining CD34 in human intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/50 in PBS/BSA and incubated with the sample for 12 hours at 4°C.

    See Abreview

参考文献

This product has been referenced in:
  • Sun JN  et al. Hepatoid adenocarcinoma of the lung without production of a-fetoprotein: A case report and review of the literature. Oncol Lett 12:189-194 (2016). IHC-P ; Human . Read more (PubMed: 27347123) »
  • Kumar P  et al. Differential Effects of Sodium Butyrate and Lithium Chloride on Rhesus Monkey Trophoblast Differentiation. PLoS One 10:e0135089 (2015). Read more (PubMed: 26266541) »

See all 4 Publications for this product

レビューと Q&A

We unfortunately cannot provide ab20147 and ab54217 as FITC conjugated antibodies because we do not custom conjugate the antibodies.

We however have the following anti CD90 FITC conjugated antibodies that may be of your interest

ab...

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Thank you for your email.

CD271: We have ab8877 which is fully tested with Sheep samples and is guaranteed to work.

CD34: ab963, ab8536, ab30375, ab30377 are the sheep specific antibodies.

CD11b: It seems ab75476, ab62817...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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投稿 May 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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