製品の概要

  • 製品名Anti-CD34 antibody [EP373Y]
    CD34 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP373Y] to CD34
  • 特異性ab81289 is not suitable for rat and mouse species in WB application.
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IP, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Dog, Human, Loxodonta Africana (African bush elephant)
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD34 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P28906

  • ポジティブ・コントロール
    • Western Blot control TF-1. IHC controls Human kidney, HUVEC cells and rat kidney. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal brain.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 41 kDa).

For unpurified use at 1/10000 - 1/100000.

ab81289 is not suitable for rat and mouse species in WB application. 

IHC-P 1/2500 - 1/5000.

For IHC antigen retrieval add – See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at  1/100 - 1/250.

ICC/IF 1/100 - 1/250.

 

IP 1/30.
IHC-Fr 1/100.
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 機能Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • 組織特異性Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • 配列類似性Belongs to the CD34 family.
  • 発生段階On early hematopoietic progenitor cells.
  • 翻訳後修飾Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Anti-CD34 antibody [EP373Y] 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Normal tonsil vessels tissue labeling CD34 with unpurified ab81289 at 1/100-1/250.

     

     

  • Immunocytochemistry/Immunofluorescence analysis of human embryonic stem cell-derived endothelial cells labeling CD34 with unpurified ab81289 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% aTriton X-100. An Alexa Fluor® 647-conjugated secondary antibody was used at a 1/400 dilution. DAPI (blue) was used as the nuclear counter stain.

  • Immunocytochemistry/Immunofluorescence analysis of HUVEC (Human umbilical vein endothelial cell line) cells labelling CD34 with purified ab81289 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain.

    Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Flow Cytometry analysis of TF-1(human erythroleukemia ) cells labeling CD34 with purified ab81289 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human angiosarcoma labeling CD34 with unpurified ab81289 at 1/100-1/250.

  • purified at 1/10000 dilution + TF-1 (Human bone marrow erythroleukemia cells) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 41 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.

  • CD34 was immunoprecipitated from TF-1 (Human bone marrow erythroleukemia cells) cells using purified ab81289 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab81289. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution. Blocking and dilution buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Normal kidney vessels tissue labeling CD34 with unpurified ab81289 at 1/100-1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Normal uterus vessels tissue labeling CD34 with unpurified ab81289 at 1/100-1/250.


     

  • IHC image of CD34 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab81289, 1/250 dilution, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CD34 antibody [EP373Y] (ab81289) 使用論文

This product has been referenced in:
  • Du M  et al. Acellular dermal matrix loading with bFGF achieves similar acceleration of bone regeneration to BMP-2 via differential effects on recruitment, proliferation and sustained osteodifferentiation of mesenchymal stem cells. Mater Sci Eng C Mater Biol Appl 70:62-70 (2017). IHC-P ; Rat . Read more (PubMed: 27770935) »
  • Hong CS  et al. Isolation of biologically active and morphologically intact exosomes from plasma of patients with cancer. J Extracell Vesicles 5:29289 (2016). WB ; Human . Read more (PubMed: 27018366) »

See all 66 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Baboon Tissue sections (Kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate, pH 6
Permeabilization No
Specification Kidney
Blocking step Background Punisher Reagent, Biocare Medicals as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Formaldehyde
Username

Morgan Thompson

Verified customer

投稿 Nov 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6.0
Permeabilization No
Specification Liver
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 06 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Permeabilization No
Specification brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 30 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Coronary artery)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: high PH DAKO S3307
Permeabilization No
Specification Coronary artery
Blocking step Dako REAL (TM) Antibody diluent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative Formaldehyde
Username

Frau Dr. Katja Eildermann

Verified customer

投稿 Aug 15 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (artery)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer pH 6.0
Permeabilization No
Specification artery
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 09 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization Yes - Commercial Wash Buffer with Tween
Specification Kidney
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Jun 16 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with Tween
Specification kidney
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Jun 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH6
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% normal buffered formalin
Username

Abcam user community

Verified customer

投稿 Mar 11 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (artery)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH 6
Permeabilization No
Specification artery
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered normal formalin
Username

Abcam user community

Verified customer

投稿 Jan 29 2016

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (artery)
Permeabilization No
Specification artery
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 28 2016

1-10 of 37 Abreviews or Q&A

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