製品の概要

  • 製品名Anti-CD34 antibody [9B10D4]
    CD34 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9B10D4] to CD34
  • アプリケーション適用あり: WB, ELISA, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Ni-NTA purified truncated recombinant CD34 expressed in E. Coli strain BL21 (DE3).

  • ポジティブ・コントロール
    • IF: Peripheral blood cells. WB: CD34 recombinant protein

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 41 kDa).
ELISA 1/10000.
ICC/IF 1/100 - 1/500.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • 組織特異性Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • 配列類似性Belongs to the CD34 family.
  • 発生段階On early hematopoietic progenitor cells.
  • 翻訳後修飾Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Anti-CD34 antibody [9B10D4] 画像

  • Overlay histogram showing Jurkat cells stained with ab54208 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54208, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG ( 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-CD34 antibody [9B10D4] (ab54208) at 1/200 dilution + Truncated CD34 recombinant protein

    Predicted band size : 41 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of peripheral blood cells using ab54208 (1/100) with FITC-IgG.
  • ab54208 at 1/1000 dilution staining CD34 in human spleen tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The DAB staining procedure was used for tissue section staining.

Anti-CD34 antibody [9B10D4] (ab54208) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Mel 30)
Specification Mel 30
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Ms. Beate Thode

Verified customer

投稿 Feb 20 2013

Thank you for your inquiry.
The immunogen used to generate ab54208 was a Ni-NTA purified truncated human recombinant CD34 expressed in E. Coli strain BL21 with the following sequence:

MLVRRGARAGPRMPRGWTALCLLSLLPSGFMSLDNNGTATPELPTQGTFSNV...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"