The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 20µl for 106 cells. A cell suspension volume of 100 µl is recommended.
ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Belongs to the CD34 family.
On early hematopoietic progenitor cells.
Highly glycosylated. Phosphorylated on serine residues by PKC.
Castro FV et al. 5T4 oncofetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype. Leukemia26:1487-98 (2012).
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