製品の概要

  • 製品名
    Anti-CD33 antibody [WM53]
    CD33 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [WM53] to CD33
  • アプリケーション
    適用あり: ICC, Flow Cyt, IP, WB, IHC-Frmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Human AML cells.

  • ポジティブ・コントロール
    • Lymphatic tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/50.
Flow Cyt 1/10 - 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. May also detects the smaller precursor protein at 40 kDa.
IHC-Fr 1/50 - 1/100.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
    • 組織特異性
      Monocytic/myeloid lineage cells.
    • 配列類似性
      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • ドメイン
      Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • 翻訳後修飾
      Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD 33 antibody
      • CD33 antibody
      • CD33 antigen (gp67) antibody
      • CD33 antigen antibody
      • CD33 molecule antibody
      • CD33_HUMAN antibody
      • FLJ00391 antibody
      • gp67 antibody
      • My9 antibody
      • Myeloid cell surface antigen CD33 antibody
      • Myeloid cell surface antigen CD33 precursor antibody
      • Myeloid differentiation antigen CD33 antibody
      • p67 antibody
      • Sialic acid binding Ig like lectin 3 antibody
      • Sialic acid binding immunoglobulin like lectin 3 antibody
      • Sialic acid-binding Ig-like lectin 3 antibody
      • SIGLEC 3 antibody
      • Siglec-3 antibody
      • SIGLEC3 antibody
      see all

    画像

    • ab30371 staining human peripheral blood monocytes by flow cytometry

    参考文献

    This product has been referenced in:

    See 1 Publication for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cell (Cytospin (human leukemia cells))
    Specification
    Cytospin (human leukemia cells)
    Fixative
    Acetone
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    投稿 May 22 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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