製品の概要

  • 製品名Anti-CD33 antibody [6C5/2]
    CD33 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6C5/2] to CD33
  • アプリケーション適用あり: IHC-P, Flow Cyt, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    CD33-Fc recombinant protein.

  • ポジティブ・コントロール
    • IHC-P: Human tonsil FFPE tissue sections

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
  • 組織特異性Monocytic/myeloid lineage cells.
  • 配列類似性Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • ドメインContains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • 翻訳後修飾Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 33 antibody
    • CD33 antibody
    • CD33 antigen (gp67) antibody
    • CD33 antigen antibody
    • CD33 molecule antibody
    • CD33_HUMAN antibody
    • FLJ00391 antibody
    • gp67 antibody
    • My9 antibody
    • Myeloid cell surface antigen CD33 antibody
    • Myeloid cell surface antigen CD33 precursor antibody
    • Myeloid differentiation antigen CD33 antibody
    • p67 antibody
    • Sialic acid binding Ig like lectin 3 antibody
    • Sialic acid binding immunoglobulin like lectin 3 antibody
    • Sialic acid-binding Ig-like lectin 3 antibody
    • SIGLEC 3 antibody
    • Siglec-3 antibody
    • SIGLEC3 antibody
    see all

Anti-CD33 antibody [6C5/2] 画像

  • IHC image of CD33 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11032, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CD33 antibody [6C5/2] (ab11032) 使用論文

This product has been referenced in:
  • Zou Z  et al. Siglecs Facilitate HIV-1 Infection of Macrophages through Adhesion with Viral Sialic Acids. PLoS One 6:e24559 (2011). Flow Cyt . Read more (PubMed: 21931755) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin melanoma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
Permeabilization No
Specification Skin melanoma
Blocking step Hydrogen peroxide as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Nov 02 2015

Thank you very much for your call yesterday and for your patience while I have been in touch with the lab regarding this antibody.

The lab scientists confirmed that the antibody cross-reacts with mouse samples, but unfortunately we don't have...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"