製品の概要

  • 製品名Anti-CD32B antibody [EP888Y]
    CD32B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP888Y] to CD32B
  • 特異性This antibody has weak cross-reactivity with CD32A and CD32C. It detects weak signal in CD32A and CD32C over-expressed lysates at the dilution of 1/200 under the exposure time of 3 minutes, but detects strong band in CD32B over-expressed lysates at the dilution of 1:2000 under the exposure time of 15 seconds.
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human CD32B (C terminal).

  • ポジティブ・コントロール
    • Daudi cell lysate, human lymph node tissue.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 49-55 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • 追加情報Is unsuitable for Flow Cyt,ICC/IF or IP.
  • ターゲット情報

    • 機能Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
    • 組織特異性Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
    • 関連疾患Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
    • 配列類似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • ドメインContains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD32 antibody
      • CD32 antigen antibody
      • CD32B antibody
      • CDw32 antibody
      • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor antibody
      • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor for antibody
      • Fc gamma receptor IIB antibody
      • Fc gamma RII antibody
      • Fc gamma RIIB antibody
      • Fc-gamma RII antibody
      • Fc-gamma RII-b antibody
      • Fc-gamma-RIIb antibody
      • FCG2 antibody
      • FCG2B_HUMAN antibody
      • Fcgr2 antibody
      • FCGR2B antibody
      • FCRII antibody
      • FcRII-b antibody
      • IGFR2 antibody
      • IgG Fc receptor II beta antibody
      • IgG Fc receptor II-b antibody
      • Low affinity immunoglobulin gamma Fc region receptor II antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
      • Ly-17 antibody
      • Lym 1 antibody
      • Lymphocyte antigen 17 antibody
      see all

    Anti-CD32B antibody [EP888Y] 画像

    • Unpurified ab45143 staining human CD32 (1/100) in human lymph node by immunohistochemistry using paraffin embedded tissue.

    • All lanes : Anti-CD32B antibody [EP888Y] (ab45143) at 1/10000 dilution (purified)

      Lane 1 : Human thyroid lysate
      Lane 2 : Daudi whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Observed band size : 40-44 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human spleen with purified ab45143 at a working dilution of 1/1000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Lanes 1, 2, 5 & 6 : Anti-CD32B antibody [EP888Y] (ab45143) at 1/200 dilution (unpurified)
      Lanes 3 - 4 : Anti-CD32B antibody [EP888Y] (ab45143) at 1/2000 dilution (unpurified)

      Lane 1 : Human CD32A overexpressed lysate
      Lane 2 : Non-transfected lysate
      Lane 3 : Human CD32B overexpressed lysate
      Lane 4 : Non-transfected lysate
      Lane 5 : Human CD32C overexpressed lysate
      Lane 6 : Non-transfected lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size : 39 kDa (why is the actual band size different from the predicted?)

      Exposure times: Lanes 1 and 2: 3 minutes, Lanes 3 and 4: 15 seconds, Lanes 5 and 6: 3 minutes.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-CD32B antibody [EP888Y] (ab45143) at 1/50000 dilution (unpurified) + Daudi cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Observed band size : 49-55 kDa (why is the actual band size different from the predicted?)
      .

    Anti-CD32B antibody [EP888Y] (ab45143) 使用論文

    This product has been referenced in:
    • Murinello S  et al. Fc? receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration. Invest Ophthalmol Vis Sci 55:247-58 (2014). IHC-P ; Human . Read more (PubMed: 24334446) »
    • Smith AJ  et al. Live simian immunodeficiency virus vaccine correlate of protection: immune complex-inhibitory Fc receptor interactions that reduce target cell availability. J Immunol 193:3126-33 (2014). Read more (PubMed: 25143442) »

    See all 11 Publications for this product

    Product Wall

    Thank you for your enquiry and your interest in our products. This antibody is very specific to FcgammaR-IIB. It will not detect R-IIA or RIIC. Although this will detect all 3 isoforms for FcgammR-IIB (1, 2 and 3). I hope this will be useful for...

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    Thank you for your enquiry.

    I can confirm that ab33515 and ab45143antibodiesare sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentrati...

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    FC gamma RIIB is not predicted to recognize FC gamma RIIA or FC gamma RIIC. Sequence homology for both isoforms is 75% in 4 residues overlapping. However, it is not guaranteed without actual testing.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"