製品の概要
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製品名Anti-CD32B antibody
CD32B 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to CD32B
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由来種Goat
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アプリケーション適用あり: WB, IHC-Pmore details
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種交差性交差種: Human
交差が予測される動物種: Cynomolgus monkey
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免疫原
Synthetic peptide:
PDALEEPDDQNRI
by a Cysteine residue linker, corresponding to C terminal amino acids 298-310 of Human CD32 (FCGR2B) -
ポジティブ･コントロール
- K562 cell lysate and U937 cell lysate. Lysate prepared from transfected HEK293 cells transiently expressing CD32B.
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特記事項
This antibody is expected to recognise all four reported isoforms (NP_001002273; NP_001002274; NP_001002275; NP_003992).
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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一次抗体 備考This antibody is expected to recognise all four reported isoforms (NP_001002273; NP_001002274; NP_001002275; NP_003992).
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab77093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). | |
| IHC-P | Use a concentration of 3 - 6 µg/ml. |
ターゲット情報
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機能Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
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組織特異性Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
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関連疾患Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
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配列類似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
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ドメインContains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
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細胞内局在Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 2213 Human
- Omim: 604590 Human
- SwissProt: P31994 Human
- Unigene: 654395 Human
- Unigene: 713617 Human
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別名
- CD32 antibody
- CD32 antigen antibody
- CD32B antibody
see all
画像
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Western blot - Anti-CD32B antibody (ab77093)Anti-CD32B antibody (ab77093) at 0.3 µg/ml + K562 lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 34 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody (ab77093)ab77093 at 3.8 µg/ml staining CD32B in Human liver by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
参考文献
ab77093 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"