製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CD320
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    fragment corresponding to C terminal amino acids 255-282 (AQERLRPLGLLVAMKESLLLSEQKTSLP) of Human CD320.

  • ポジティブ・コントロール
    • Human lymph node tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin.
  • 組織特異性
    Expressed abundantly on follicular dendritic cells (FDCs).
  • 関連疾患
    Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC) [MIM:613646]; also called methylmalonic aciduria due to transcobalamin receptor defect. MMATC is a metabolic disorder characterized by increased blood C3-acylcarnitine levels, elevated methylmalonate and homocysteine, and low uptake of transcobalamin-bound cobalamin, but normal conversion to adenosylcobalamin and methylcobalamin. Plasma vitamin B12 and total homocysteine are normal.
  • 配列類似性
    Contains 2 LDL-receptor class A domains.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 8D6 antibody
    • 8D6 antigen antibody
    • 8D6A antibody
    • CD320 antibody
    • CD320 antigen antibody
    • CD320_HUMAN antibody
    • FDC-signaling molecule 8D6 antibody
    • FDC-SM-8D6 antibody
    • TCblR antibody
    • Transcobalamin receptor antibody
    see all

画像

  • ab121433 at a 1/50 dilution staining CD320 in paraffin-embedded Human lymph node tissue by Immunohistochemistry.

プロトコール

参考文献

This product has been referenced in:
  • Fowler R  et al. Uptake and transport of B12-conjugated nanoparticles in airway epithelium. J Control Release 172:374-81 (2013). ICC/IF ; Human . Read more (PubMed: 24008152) »

See 1 Publication for this product

レビューと Q&A

Abreviews
Application
Western blot
Sample
Human Purified protein (Hep G2 (Liver))
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (Stacking gel (10%), Resolving gel (10%))
Loading amount
20 µg
Treatment
10 concentrations of Vitamin B12 (500nM, 400nM, 200nM, 100nM, 50nM, 20nM, 10nM, 1nM, 100pM and 25pM) for 10 days
Specification
Hep G2 (Liver)
Blocking step
(agent) for 12 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 4°C
Username

Joseph Boachie

Verified customer

投稿 Aug 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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