製品の概要

  • 製品名Anti-CD32 antibody [AT10]
    CD32 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AT10] to CD32
  • 特異性AT10 recognises the human CD32 antigen, a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. According to the CD32 Cluster workshop (Leucocyte Typing V, pp823, pp828), clone AT10 reacts equally well with both Fc gamma RII a and b-expressing cells.
  • アプリケーション適用あり: IP, Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog, Rhesus monkey
  • 免疫原

    Human K562 cell line

  • ポジティブ・コントロール
    • This antibody gave a positive signal in K562 cells in Flow Cytometry.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 0.02 mg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/500 - 1/1000.

ターゲット情報

  • 機能Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
  • 組織特異性Found on monocytes, neutrophils and eosinophil platelets.
  • 配列類似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD32 antibody
    • CD32A antibody
    • CD32B antibody
    • CD32C antibody
    • CDw32 antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIa receptor for (CD32) antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIb receptor for (CD32) antibody
    • Fc fragment of IgG low affinity IIc receptor antibody
    • Fc fragment of IgG low affinity IIc receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
    • Fc gamma receptor IIB antibody
    • Fc gamma receptor IIC antibody
    • Fc gamma RII a antibody
    • Fc gamma RII b antibody
    • Fc gamma RII c antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIIc antibody
    • Fc receptor, IgG, low affinity IIb antibody
    • Fc-gamma RII-a antibody
    • Fc-gamma RII-b antibody
    • Fc-gamma RII-c antibody
    • Fc-gamma-RIIa antibody
    • FC-gamma-RIIB antibody
    • Fc-GAMMA-RIIC antibody
    • Fc[g]RII antibody
    • FCG2 antibody
    • FCG2A_HUMAN antibody
    • FcGR antibody
    • FCGR2 antibody
    • FCGR2A antibody
    • FCGR2A1 antibody
    • FCGR2B antibody
    • FCGR2C antibody
    • Fcgr3 antibody
    • Fcgr3a antibody
    • FcgRII antibody
    • Fcr-2 antibody
    • Fcr-3 antibody
    • FcRII a antibody
    • FCRII antibody
    • FcRII b antibody
    • FcRII c antibody
    • FcRII-a antibody
    • FcRIIC antibody
    • IGFR2 antibody
    • IgG Fc receptor II a antibody
    • IgG Fc receptor II b antibody
    • IgG Fc receptor II beta antibody
    • IgG Fc receptor II c antibody
    • IgG Fc receptor II-a antibody
    • IgG Fc receptor II-b antibody
    • IgG Fc receptor II-c antibody
    • Immunoglobulin G Fc receptor II antibody
    • Immunoglobulin G Fc receptor II c antibody
    • Low affinity immunoglobulin gamma Fc region receptor II antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-c antibody
    • Ly-17 antibody
    • Ly-m20 antibody
    • LyM-1 antibody
    • Lymphocyte antigen 17 antibody
    see all

Anti-CD32 antibody [AT10] 画像

  • Overlay histogram showing K562 cells stained with ab23336 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed K562 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD32 antibody [AT10] (ab23336) 使用論文

This product has been referenced in:
  • Moutsopoulos NM  et al. Tonsil epithelial factors may influence oropharyngeal human immunodeficiency virus transmission. Am J Pathol 171:571-9 (2007). Read more (PubMed: 17620369) »
  • Wang JP  et al. Cutting Edge: Antibody-mediated TLR7-dependent recognition of viral RNA. J Immunol 178:3363-7 (2007). Read more (PubMed: 17339429) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"