Anti-CD31 抗体 [SP38] - C-terminal, prediluted (ab101686)

製品の概要

  • 製品名
    Anti-CD31 antibody [SP38] - C-terminal, prediluted
    CD31 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP38] to CD31 - C-terminal, prediluted
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Mouse CD31 (C terminal).
    Database link: Q08481

  • ポジティブ・コントロール
    • IHC-P: Human esophagus, cervix, colon adenocarcinoma, colon, heart, liver hepatocellular carcinoma, liver, endometrial adenocarcinoma, lung adenocarcinoma, lung, endometrium and tonsil tissue.

アプリケーション

Our Abpromise guarantee covers the use of ab101686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1.

Ready to use.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Incubate for 10 minutes at room temperature.

ターゲット情報

  • 機能
    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 組織特異性
    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 配列類似性
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • ドメイン
    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻訳後修飾
    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 細胞内局在
    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

画像

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD31 with ab101686 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervix tissue stained for CD31 with ab101686 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human heart tissue stained for CD31 with ab101686 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human endometrium tissue stained for CD31 with ab101686 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for CD31 with ab101686 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for CD31 with ab101686 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung tissue stained for CD31 with ab101686 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for CD31 with ab101686 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD31 with ab101686 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human esophagus tissue stained for CD31 with ab101686 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD31 with ab101686 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for CD31 with ab101686 in immunohistochemical analysis.

参考文献

This product has been referenced in:
  • Clement M  et al. Upholding the T cell immune-regulatory function of CD31 inhibits the formation of T/B immunological synapses in vitro and attenuates the development of experimental autoimmune arthritis in vivo. J Autoimmun 56:23-33 (2015). Read more (PubMed: 25277651) »
  • Cañadas I  et al. Targeting epithelial-to-mesenchymal transition with Met inhibitors reverts chemoresistance in small cell lung cancer. Clin Cancer Res 20:938-50 (2014). Read more (PubMed: 24284055) »

See all 4 Publications for this product

レビューと Q&A

Vielen Dank für die zwei Sequenzen von Lachs (Salmo salar).
Ich habe beide mit den Immunogenen aller unserer CD31 Antikörper aus dem Kaninchen verglichen.
Leider hat keine Immunogensequenz auch nur annähernd eine Homologie grösser als ein paa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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