製品の概要

  • 製品名
    Anti-CD31 antibody [P2B1]
    CD31 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [P2B1] to CD31
  • アプリケーション
    適用あり: ICC/IF, IHC - Wholemount, WB, IPmore details
    適用なし: IHC-Fr or IHC-P
  • 種交差性
    交差種: Mouse, Human
    非交差種: Cow
  • 免疫原

    Tissue, cells or virus corresponding to Human CD31.

  • 特記事項

    ab24590 activates the binding of human T-lymphocytes to endothelial cells.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/1000.
IHC - Wholemount 1/100.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 83 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

IP 1/1000.
  • 追加情報
    Is unsuitable for IHC-Fr or IHC-P.
  • ターゲット情報

    • 機能
      Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
    • 組織特異性
      Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
    • 配列類似性
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • ドメイン
      The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
    • 翻訳後修飾
      Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
    • 細胞内局在
      Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Adhesion molecule antibody
      • CD31 antibody
      • CD31 antigen antibody
      • CD31 EndoCAM antibody
      • EndoCAM antibody
      • FLJ34100 antibody
      • FLJ58394 antibody
      • GPIIA antibody
      • GPIIA' antibody
      • PECA1 antibody
      • PECA1_HUMAN antibody
      • Pecam 1 antibody
      • PECAM 1 CD31 EndoCAM antibody
      • PECAM antibody
      • PECAM-1 antibody
      • Pecam1 antibody
      • Platelet and endothelial cell adhesion molecule 1 antibody
      • Platelet endothelial cell adhesion molecule antibody
      • Platelet/endothelial cell adhesion molecule 1 antibody
      see all

    画像

    • Positive immunocytochemical staining of human primary choroidal endothelial cells using ab24590 at a dilution of 1/100. The tissue was fixed with paraformaldehyde and the antibody was incubated for 3 hours. An Alexa Fluor® 488 goat anti-mouse antibody was used as a secondary antibody.

      See Abreview

    • Images of isolated capillaries triple labeled for EC marker protein CD31 (red channel), PC marker protein NG-2 (green channel), and Hoechst for nuclei (blue channel).

      Cochlear stria vascularis capillaries were isolated from 6-week-old CBA/CaJ mice. The capillary isolation was achieved with a special procedure we developed and named the “sandwich-dissociation” method. The tissue samples were fixed in 4% paraformaldehyde at 4°C for 2 h, washed in PBS for 30 min, permeabilized in 0.5% Triton X-100, then immunoblocked in a solution of 10% goat serum in 1% bovine albumin in PBS for 30 min. The specimens were incubated with primary antibodies and secondary antibodies (goat Alexa Fluo 488-conjugated anti-mouse and anti-rabbit IgG antibodies).

    • ab24590 staining CD31 in the HEK 293T cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton and blocked with 3% BSA for 1 hour at RT. Samples were incubated with primary antibody (1/50 in PBS + 3% BSA) for 1 hour. A Alexa Fluor® 555-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody (1/500).

      See Abreview

    • All lanes : Anti-CD31 antibody [P2B1] (ab24590) at 5 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) WCL
      Lane 2 : THP1 (Human acute monocytic leukemia cell line) WCL
      Lane 3 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
      Lane 4 : Human Platelets

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size : 83 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 4 minutes

      We recommend using 3% milk as the blocking agent for Western blot.

    参考文献

    This product has been referenced in:
    • Zhang H  et al. Pigment epithelium-derived factor attenuates myocardial fibrosis via inhibiting Endothelial-to-Mesenchymal Transition in rats with acute myocardial infarction. Sci Rep 7:41932 (2017). ICC/IF ; Rat . Read more (PubMed: 28167820) »
    • Jin YG  et al. Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-ß1/Smad2-Mediated Endothelial-to-Mesenchymal Transition. PPAR Res 2017:2647129 (2017). WB, IF ; Mouse . Read more (PubMed: 28638404) »

    See all 43 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human Lung)
    Permeabilization
    No
    Specification
    Human Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Jim Hsiao

    Verified customer

    投稿 Sep 14 2017

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Mouse lung tissue)
    Permeabilization
    No
    Specification
    Mouse lung tissue
    Blocking step
    Dako non-serum block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Jim Hsiao

    Verified customer

    投稿 Jun 08 2017

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Pig Cell lysate - whole cell (PAE cells)
    Specification
    PAE cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 21 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Fish Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM citrate pH6.0
    Permeabilization
    Yes - Triton X
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Dec 24 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human fibromatosis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Permeabilization
    Yes - Tween-20
    Specification
    Human fibromatosis
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Dec 15 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Permeabilization
    Yes - Tween-20
    Specification
    mouse liver
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Dec 08 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample
    Human Cell (HEK 293T)
    Specification
    HEK 293T
    Permeabilization
    Yes - Triton 1%
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Nov 18 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Peripheral blood lymphocyte (PBL))
    Specification
    Peripheral blood lymphocyte (PBL)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    投稿 Oct 22 2014

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rat Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 22 2014

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 22 2014

    1-10 of 40 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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