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Mouse t.end.1 cells (polyoma middle T (PmT)-transformed EC)
Our Abpromise guarantee covers the use of ab7388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
No fixation or permeabilization needed. Epitope is sensitive to trypsin!
|ICC/IF||Use at an assay dependent concentration. Please see reviews for fixative recommendations. Certain users find acetone fixation necessary while others find 4% PFA necessary. In addition, found to work at 1/1000 dilution.|
|IP||Use at an assay dependent concentration.|
Acetone (-20°C) fixation for 10 minutes at room temperature is recommended.
Immunohistochemical analysis of frozen mouse skin tissue, staining CD31 with ab7388.
Tissue was fixed with acetone and blocked with 2% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300 in 1% BSA) for 4 hours at 20°C. An Alexa Fluor® 488-conjugated goat anti-rat monoclonal IgG (1/500) was used as the secondary antibody.
Immunohistochemistry (Frozen sections) analysis of mouse embryo transverse tissue section labeling CD31 with ab7388 at 1/1000 dilution. Tissue sections were fixed with paraformaldehyde and permeabilized with PBST (PBS / 0.5% v/v Triton X-100). Tissue was blocked for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA + 5% donkey serum + PBST) for 12 hours at 4°C. A polyclonal goat anti-rat Alex Fluor® was used as the secondary antibody at 1/1000 dilution. This is a transverse section of E10.5 mouse embryo. CD31 expresses in endothelium cells which locates in the surface of dorsal aorta and small vessels.
Flow Cytometry analysis of bEND3 (mouse brain endothelial cell line) cells labeling CD31 with ab7388 at 10 µg/ml concentration. 106 cells were used per sample. No fixation or permeabilization needed. A R-PE labelled goat anti-rat IgG (H+L) was used as the secondary antibody.
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