製品の概要

  • 製品名Anti-CD31 antibody [MEC 7.46]
    CD31 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [MEC 7.46] to CD31
  • 特異性The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cell presents on all mouse blood vessels both in normal and inflamed or tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.
  • アプリケーション適用あり: Flow Cyt, ICC/IF, IP, IHC-Frmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse
  • 免疫原

    Mouse t.end.1 cells (polyoma middle T (PmT)-transformed EC)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

No fixation or permeabilization needed. Epitope is sensitive to trypsin!
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. Please see reviews for fixative recommendations. Certain users find acetone fixation necessary while others find 4% PFA necessary. In addition, found to work at 1/1000 dilution.
IP Use at an assay dependent concentration.
IHC-Fr 1/10.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
    • 組織特異性Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
    • 配列類似性Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • ドメインThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
    • 翻訳後修飾Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
    • 細胞内局在Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Adhesion molecule antibody
      • CD31 antibody
      • CD31 antigen antibody
      • CD31 EndoCAM antibody
      • EndoCAM antibody
      • FLJ34100 antibody
      • FLJ58394 antibody
      • GPIIA antibody
      • GPIIA' antibody
      • PECA1 antibody
      • PECA1_HUMAN antibody
      • Pecam 1 antibody
      • PECAM 1 CD31 EndoCAM antibody
      • PECAM antibody
      • PECAM-1 antibody
      • Pecam1 antibody
      • Platelet and endothelial cell adhesion molecule 1 antibody
      • Platelet endothelial cell adhesion molecule antibody
      • Platelet/endothelial cell adhesion molecule 1 antibody
      see all

    Anti-CD31 antibody [MEC 7.46] 画像

    • Immunohistochemical analysis of frozen mouse skin tissue, staining CD31 with ab7388.
      Tissue was fixed with acetone and blocked with 2% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300 in 1% BSA) for 4 hours at 20°C. An Alexa Fluor® 488-conjugated goat anti-rat monoclonal IgG (1/500) was used as the secondary antibody.

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    Anti-CD31 antibody [MEC 7.46] (ab7388) 使用論文

    This product has been referenced in:
    • Fu J  et al. Comparison of Glomerular and Podocyte mRNA Profiles in Streptozotocin-Induced Diabetes. J Am Soc Nephrol 27:1006-14 (2016). Read more (PubMed: 26264855) »
    • Li M  et al. Targeting of cancer-associated fibroblasts enhances the efficacy of cancer chemotherapy by regulating the tumor microenvironment. Mol Med Rep 13:2476-84 (2016). IHC . Read more (PubMed: 26846566) »

    See all 39 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Adipose tissue)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citric acid, 55'C, 20min
    Permeabilization Yes - PBS/ 0.1% v/v Triton X-100 (Sigma)
    Specification Adipose tissue
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Mr. Bongju Park

    Verified customer

    投稿 Dec 01 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (mouse skin)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako target retrieval solution
    Permeabilization No
    Specification mouse skin
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 19 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (E10.5 mouse embryos transverse section)
    Specification E10.5 mouse embryos transverse section
    Fixative Paraformaldehyde
    Permeabilization Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
    Blocking step NOVOCASTRA protein block as blocking agent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 May 13 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (mouse skin)
    Specification mouse skin
    Fixative Acetone
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 09 2013

    I now have some further information to share in regards to the anti-CD31 antibody [MEC 7.46] (ab7388).

    I have been in contact with the reviewer and they have confirmed that they did indeed use an anti-mouse HRP DAKO kit to detect this rat ant...

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    Thank you for your response and for clarifying the cell types used in the experiments.    I understand now that bEnd3 cells (immortalized mouse brain endothelial cell line) were stained for CD31. The protocol looks absolutely fine and it you have an...

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    Thank you for your response. Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem. 1)  Sample: Could you specify the samples used ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (lung tissue)
    Specification lung tissue
    Fixative Paraformaldehyde
    Permeabilization Yes - MeOH
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 10 2007

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. This antibody has not been tested using immunohistochemistry using paraffin sections. Indeed we have received various enquiries associated wit...

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    Thank you for your enquiry and for forwarding your detailed protocol. We would like to confirm that Ab7388 recognizes mouse CD31 and it is suitable for imminohistochemical staining on frozen sections. However, it has not been tested on fomalin-fixe...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"