製品の概要

  • 製品名
    Anti-CD31 antibody [JC/70A]
    CD31 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [JC/70A] to CD31
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human, Cynomolgus monkey
  • 免疫原

    Membrane preparation of a spleen from a patient with hairy cell leukaemia.

  • ポジティブ・コントロール
    • IHC-P: FFPE Human Tonsil normal. ICC-IF: HUVEC cells.

アプリケーション

Our Abpromise guarantee covers the use of ab9498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20618694
Flow Cyt 1/20.

Paraformaldehyde or methanol fixed cells (also see PMID: 20618694).

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 82 kDa).
IHC-P 1/10 - 1/25. 30-60mins at RT (ABC method). Biopsy specimens should be fixed in periodate-lysine-paraformaldehyde solution or 10% formalin solution. Perform enzymatic antigen retrieval (pepsin or 0.1% trypsin solution at 37°C for 60 min (see reference by Moriyama M et al) before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 組織特異性
    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 配列類似性
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • ドメイン
    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻訳後修飾
    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 細胞内局在
    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

Anti-CD31 antibody [JC/70A] 画像

  • Overlay histogram showing Jurkat cells stained with ab9498 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9498, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • ab9498 staining human tonsil by IHC-P.

  • ab9498 stained in HVEC cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9498 at 5 µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150080 (pseudo-colored red) and ab150117 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature

  • ab9498 staining CD31 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.
  • ab9498 at 1/100 staining human lymph node tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat based antigen retrieval step was performed. The tissue was then blocked with serum and incubated with ab9498 overnight. An HRP conjugated goat antibody was used as the secondary.

    See Abreview

  • Formalin fixed paraffin embedded human tonsil stained with CD31, using ABC and AEC chromagen.

Anti-CD31 antibody [JC/70A] (ab9498) 使用論文

This product has been referenced in:
  • Kim MJ  et al. Thyroid-Related Protein Expression in the Human Thymus. Int J Endocrinol 2017:8159892 (2017). Read more (PubMed: 28386277) »
  • Schuhmann MK  et al. Blocking of platelet glycoprotein receptor Ib reduces "thrombo-inflammation" in mice with acute ischemic stroke. J Neuroinflammation 14:18 (2017). Read more (PubMed: 28109273) »

See all 38 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (Heart)
Permeabilization
No
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

投稿 Sep 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Saphenous Vein)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
Yes - 0.3% Hydrogen Peroxide
Specification
Saphenous Vein
Blocking step
Casein in PBS as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Formaldehyde
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Verified customer

投稿 Apr 29 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Synovial membrane)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Protease K
Permeabilization
No
Specification
Synovial membrane
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Formaldehyde
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Verified customer

投稿 Apr 04 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain)
Specification
brain
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投稿 Mar 03 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rhesus monkey Cell (chorioretinal endothelial cell)
Permeabilization
No
Gating Strategy
size and granularity
Specification
chorioretinal endothelial cell
Preparation
Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with 0.05% Trypsin-EDTA
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation
Paraformaldehyde
Username

Andrea Cabrera

Verified customer

投稿 Feb 06 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Ovary)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate 10 mM, pH 6.0
Permeabilization
Yes - 0.025% Triton X-100
Specification
Ovary
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

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Verified customer

投稿 Sep 27 2016

Application
Immunocytochemistry
Sample
Human Cultured Cells (Primary Human Internal Thoracic Artery Endothelia)
Permeabilization
Yes - Cold Methanol
Specification
Primary Human Internal Thoracic Artery Endothelia
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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Verified customer

投稿 Sep 25 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen retrieval solution pH 9 of DAKO
Permeabilization
No
Specification
Lung
Blocking step
Fetal calf serum (filtered) as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Username

Dr. Thibault Geinoz

Verified customer

投稿 Jun 27 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Endothelial cells (PAEC))
Specification
Endothelial cells (PAEC)
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Thibault Geinoz

Verified customer

投稿 Nov 21 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (skin)
Antigen retrieval step
None
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 04 2014

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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