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Our Abpromise guarantee covers the use of ab9498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20618694|
Paraformaldehyde or methanol fixed cells (also see PMID: 20618694).
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 82 kDa).|
|IHC-P||1/10 - 1/25. 30-60mins at RT (ABC method). Biopsy specimens should be fixed in periodate-lysine-paraformaldehyde solution or 10% formalin solution. Perform enzymatic antigen retrieval (pepsin or 0.1% trypsin solution at 37°C for 60 min (see reference by Moriyama M et al) before commencing with IHC staining protocol.|
ab9498 staining human tonsil by IHC-P.