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Tissue/ cell preparation: Mouse leukocyte cell line 32D.
This antibody inhibits the aggregation of L cells transfected with a variant form of CD31.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab24960 in the following tested applications.
|Flow Cyt||Use 1µg for 106 cells.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration.|
Flow cytometry image of ab24960 staining mouse embryonic stem cells after trypsinization from tissue culture plastic. Live cells were gated using forward and side scatter. The primary antibody was diluted 1200 and incubated with the sample for 1 hr at 4°C. A PE-Cy5® Streptavidin fusion protein at a 1/250 dilution, was used instead of a secondary antibody.