製品の概要

  • 製品名Anti-CD300 antibody [MEM-260]
    CD300 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-260] to CD300
  • 特異性ab53317 recognizes CD300a, (Customer feedback suggests it also cross-reacts with CD300c, however we have not yet tested it for CD300c). The antibody MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    HPB human lymphoid leukemia cell line.

  • ポジティブ・コントロール
    • Positive material: HPB cell line. Negative material: Jurkat human cell line, Raji human cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from hybridoma culture supernatant by protein-A affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名MEM-260
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 35 kDa. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Flow Cyt Use a concentration of 2 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Acts as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation.
  • 組織特異性Highly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocytes and monocyte-related cells.
  • 配列類似性Belongs to the CD300 family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾Phosphorylated on tyrosine.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD300 antigen like family member antibody
    • CD300 antigen-like family member F antibody
    • CD300 molecule like family member f antibody
    • CD300f antibody
    • CD300LF antibody
    • CLM antibody
    • CLM-1 antibody
    • CLM1 antibody
    • CLM1_HUMAN antibody
    • CMRF35-like molecule 1 antibody
    • IGSF antibody
    • IgSF13 antibody
    • Immune receptor expressed on myeloid cells 1 antibody
    • immune receptor expressed on myeloid cells antibody
    • immunoglobin superfamily member antibody
    • Immunoglobulin superfamily member 13 antibody
    • Immunoglobulin superfamily member antibody
    • Inhibitory receptor IREM1 antibody
    • IREM antibody
    • IREM-1 antibody
    • IREM1 antibody
    • Nepmucin antibody
    • NK inhibitory receptor antibody
    • NKIR antibody
    • TREM antibody
    • triggering receptors expressed on myeloid cells antibody
    • UNQ3105/PRO10111 antibody
    see all

Anti-CD300 antibody [MEM-260] (ab53317) 使用論文

ab53317 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab53317.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"