The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 19 kDa).
1/250 - 1/500.
追加情報Is unsuitable for Flow Cyt or IHC-P.
機能Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
関連疾患Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
配列類似性Belongs to the CD3Z/FCER1G family. Contains 3 ITAM domains.
ドメインThe ITAM domains mediate interaction with SHB.
翻訳後修飾Phosphorylated on Tyr residues after T-cell receptor triggering.
Western blot - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab68235)
All lanes : Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab68235) at 1/1000 dilution
Lane 1 : Untreated Jurkat cells whole cell lysates Lane 2 : Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates Lane 3 : Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution