Anti-CD3 zeta (phospho Y142) 抗体 (ab59175)

製品の概要

  • 製品名
    Anti-CD3 zeta (phospho Y142) antibody
    CD3 zeta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD3 zeta (phospho Y142)
  • 特異性
    Detects endogenous levels of CD3 zeta only when phosphorylated at tyrosine 142. Recognises human phosphorylated at tyrosine 142 and mouse phosphorylated at tyrosine 143.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide (Human) around the phosphorylation site of tyrosine 142 (GLYPQG)

  • ポジティブ・コントロール
    • Jurkat cell extract treated with UV

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ELISA 1/5000.

ターゲット情報

  • 機能
    Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
  • 関連疾患
    Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
  • 配列類似性
    Belongs to the CD3Z/FCER1G family.
    Contains 3 ITAM domains.
  • ドメイン
    The ITAM domains mediate interaction with SHB.
  • 翻訳後修飾
    Phosphorylated on Tyr residues after T-cell receptor triggering.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • CD247 antibody
    • CD247 antigen antibody
    • Cd247 molecule antibody
    • Cd3 antibody
    • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
    • CD3 antigen, zeta subunit antibody
    • CD3-eta antibody
    • CD3H antibody
    • CD3Q antibody
    • Cd3z antibody
    • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
    • CD3Z_HUMAN antibody
    • CD3zeta antibody
    • CD3zeta chain antibody
    • MGC140430 antibody
    • T cell receptor T3 zeta chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 zeta chain antibody
    • T-cell surface glycoprotein CD3 zeta chain antibody
    • T3z antibody
    • TCR zeta chain antibody
    • TCRk antibody
    • TCRZ antibody
    • TCRzeta antibody
    see all

画像

  • All lanes : Anti-CD3 zeta (phospho Y142) antibody (ab59175) at 1/500 dilution

    Lane 1 : Jurkat cells treated with UV (15 mins)
    Lane 2 : Jurkat cells treated with UV (15 mins) with phosphopeptide


    Predicted band size : 23 kDa
    Observed band size : 23 kDa

プロトコール

参考文献

ab59175 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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