FITC Anti-CD3 zeta 抗体 [H146-968] (ab91493)
Key features and details
- FITC Armenian hamster monoclonal [H146-968] to CD3 zeta
- Suitable for: Flow Cyt (Intra)
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-CD3 zeta antibody [H146-968]
CD3 zeta 一次抗体 製品一覧 -
製品の詳細
FITC Armenian hamster monoclonal [H146-968] to CD3 zeta -
由来種
Armenian hamster -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Sheep, Rabbit, Cow, Pig, Xenopus laevis -
免疫原
Synthetic peptide corresponding to Mouse CD3 zeta aa 150-250.
Database link: P20963 -
ポジティブ・コントロール
- Flow Cyt: Murine splenocytes
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特記事項
Purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 8.00
Preservative: 0.097% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading...
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精製度
Size exclusion -
特記事項(精製)
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
H146-968 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab91493の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use a concentration of 1 - 4 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
特記事項 |
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Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
ターゲット情報
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機能
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. -
関連疾患
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. -
配列類似性
Belongs to the CD3Z/FCER1G family.
Contains 3 ITAM domains. -
ドメイン
The ITAM domains mediate interaction with SHB. -
翻訳後修飾
Phosphorylated on Tyr residues after T-cell receptor triggering. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281056 Cow
- Entrez Gene: 12503 Mouse
- Entrez Gene: 397302 Pig
- Entrez Gene: 100008617 Rabbit
- Entrez Gene: 25300 Rat
- SwissProt: P24161 Mouse
- SwissProt: Q9XSJ9 Pig
- SwissProt: Q9TUF8 Rabbit
see all -
別名
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
画像
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Intracellular staining flow cytometric pattern of murine splenocytes stained with ab91493: anti-cd3 zeta FITC antibody with a concentration of 3µg/ml in the sample
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Murine splenocytes mulitcolour intracellular staining flow cytometric pattern using 3µg/ml ab91493 and anti-human CD3 APC antibody with 10µl reagent per 100µl peripheral whole blood
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Flow cytometric intracellular staining analysis of murine splenocyte supension using anti-CD3 zeta FITC antibody (ab91493) sample concentation of of 3µg/ml. There is separation between the CD3 positive CD3 zeta positive splenocytes (red) and CD3 negative CD3 zeta negative splenocytes in black.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab91493 は 2 報の論文で使用されています。
- Pieren DKJ et al. Response kinetics reveal novel features of ageing in murine T cells. Sci Rep 9:5587 (2019). PubMed: 30944406
- Brazin KN et al. The T Cell Antigen Receptor α Transmembrane Domain Coordinates Triggering through Regulation of Bilayer Immersion and CD3 Subunit Associations. Immunity 49:829-841.e6 (2018). PubMed: 30389415