Anti-CD3 zeta 抗体 [H146-968] - BSA and Azide free (ab119827)
Key features and details
- Armenian hamster monoclonal [H146-968] to CD3 zeta - BSA and Azide free
- Suitable for: ICC, IP, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-CD3 zeta antibody [H146-968] - BSA and Azide free
CD3 zeta 一次抗体 製品一覧 -
製品の詳細
Armenian hamster monoclonal [H146-968] to CD3 zeta - BSA and Azide free -
由来種
Armenian hamster -
アプリケーション
適用あり: ICC, IP, WB, Flow Cytmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Chicken, Pig -
免疫原
Synthetic peptide corresponding to Mouse CD3 zeta aa 150-250.
Database link: P20963 -
ポジティブ・コントロール
- WB: Murine splenocytes.
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特記事項
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
ab119827 is purified from cell culture supernatant by protein A affinity chromatography and 0.2 µm filter sterilized. Purity is > 95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
H146-968 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119827の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC | (1) |
Use at an assay dependent concentration.
|
IP |
Use at an assay dependent concentration.
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|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 19 kDa.
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|
Flow Cyt |
Use at an assay dependent concentration.
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 19 kDa. |
Flow Cyt
Use at an assay dependent concentration. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. -
関連疾患
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. -
配列類似性
Belongs to the CD3Z/FCER1G family.
Contains 3 ITAM domains. -
ドメイン
The ITAM domains mediate interaction with SHB. -
翻訳後修飾
Phosphorylated on Tyr residues after T-cell receptor triggering. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 395911 Chicken
- Entrez Gene: 919 Human
- Entrez Gene: 12503 Mouse
- Entrez Gene: 397302 Pig
- Entrez Gene: 100008617 Rabbit
- Entrez Gene: 25300 Rat
- Omim: 186780 Human
- SwissProt: P20963 Human
see all -
別名
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
画像
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All lanes : Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (ab119827)
Lane 1 : Murine splenocytes
Lane 2 : MOLT-4 cell line
Secondary
Lane 1 : conjugated goat-anti-armenian hamster
Lane 2 : Alexa Fluor® 790-conjugated goat-anti-armenian hamster
Performed under reducing conditions.
Predicted band size: 19 kDa -
Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (ab119827) at 2 µg/ml + Murine Splenocytes
Performed under reducing conditions.
Predicted band size: 19 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab119827 は 7 報の論文で使用されています。
- Lei X et al. A Pan-Histone Deacetylase Inhibitor Enhances the Antitumor Activity of B7-H3-Specific CAR T Cells in Solid Tumors. Clin Cancer Res 27:3757-3771 (2021). PubMed: 33811153
- Wei J et al. PD-1 silencing impairs the anti-tumor function of chimeric antigen receptor modified T cells by inhibiting proliferation activity. J Immunother Cancer 7:209 (2019). PubMed: 31391096
- Jelencic V et al. NK cell receptor NKG2D sets activation threshold for the NCR1 receptor early in NK cell development. Nat Immunol 19:1083-1092 (2018). PubMed: 30224819
- Iida T et al. Regulation of cell surface expression of CTLA-4 by secretion of CTLA-4-containing lysosomes upon activation of CD4+ T cells. J Immunol 165:5062-8 (2000). PubMed: 11046036
- Kosugi A et al. Translocation of tyrosine-phosphorylated TCRzeta chain to glycolipid-enriched membrane domains upon T cell activation. Int Immunol 11:1395-401 (1999). PubMed: 10464160
- Bäckström BT et al. Signaling efficiency of the T cell receptor controlled by a single amino acid in the beta chain constant region. J Exp Med 186:1933-8 (1997). PubMed: 9382891
- Rozdzial MM et al. Developmental regulation of the TCR zeta-chain. Differential expression and tyrosine phosphorylation of the TCR zeta-chain in resting immature and mature T lymphocytes. J Immunol 153:1563-80 (1994). PubMed: 7519206