The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報 IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol with 10 mM Citrate buffer, pH 6.0.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
関連疾患Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
配列類似性Belongs to the CD3Z/FCER1G family. Contains 3 ITAM domains.
ドメインThe ITAM domains mediate interaction with SHB.
翻訳後修飾Phosphorylated on Tyr residues after T-cell receptor triggering.
IHC image of ab17061 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17061, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-CD3 zeta antibody (ab17061) 使用論文
This product has been referenced in:
Guzik TJ et al. Role of the T cell in the genesis of angiotensin II induced hypertension and vascular dysfunction. J Exp Med204:2449-60 (2007).
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