製品の概要

  • 製品名Anti-CD3 zeta antibody
    CD3 zeta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD3 zeta
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Pig
  • 免疫原

    Synthetic peptide:

    DGILFIYGVILTALFLRVK

    , corresponding to amino acids 36-54 of Human CD3 zeta.

  • ポジティブ・コントロール
    • Tonsil

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab17061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol with 10 mM Citrate buffer, pH 6.0.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
    • 関連疾患Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
    • 配列類似性Belongs to the CD3Z/FCER1G family.
      Contains 3 ITAM domains.
    • ドメインThe ITAM domains mediate interaction with SHB.
    • 翻訳後修飾Phosphorylated on Tyr residues after T-cell receptor triggering.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • CD247 antibody
      • CD247 antigen antibody
      • Cd247 molecule antibody
      • Cd3 antibody
      • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
      • CD3 antigen, zeta subunit antibody
      • CD3-eta antibody
      • CD3H antibody
      • CD3Q antibody
      • Cd3z antibody
      • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
      • CD3Z_HUMAN antibody
      • CD3zeta antibody
      • CD3zeta chain antibody
      • MGC140430 antibody
      • T cell receptor T3 zeta chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 zeta chain antibody
      • T-cell surface glycoprotein CD3 zeta chain antibody
      • T3z antibody
      • TCR zeta chain antibody
      • TCRk antibody
      • TCRZ antibody
      • TCRzeta antibody
      see all

    Anti-CD3 zeta antibody 画像

    • IHC image of ab17061 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17061, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-CD3 zeta antibody (ab17061) 使用論文

    This product has been referenced in:
    • Guzik TJ  et al. Role of the T cell in the genesis of angiotensin II induced hypertension and vascular dysfunction. J Exp Med 204:2449-60 (2007). Read more (PubMed: 17875676) »

    See 1 Publication for this product

    Product Wall

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Unseres Wissens nach wurde ab17061 bisher noch nicht inIHC-Fr getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angeb...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"