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The details of the immunogen for this antibody are not available.
Porcine CD3e is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. ab25535 PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.
Our Abpromise guarantee covers the use of ab25535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Celluar Activation||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.2µg for 106 cells. ab67435-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|