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The details of the immunogen for this antibody are not available.
In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.
Our Abpromise guarantee covers the use of ab25380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|IP||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.
ab25380 can be used for in vitro depletion of CD3 positive cells.
|Cellular Activation||Use at an assay dependent concentration.
ab25380 can activate primed and naive T cell in vitro (accessory cells must be provided).
BALB/c lymph node cells were double stained with ab25380 and with anti-IL2RB (ab24943) at 1µg/106 cells. Small lymphocytes were gated and analyzed on a flow cytometer.