製品の概要

  • 製品名
    Anti-CD3 epsilon antibody [C363.29B] (PE/Cy5®)
    CD3 epsilon 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [C363.29B] to CD3 epsilon (PE/Cy5®)
  • 標識
    PE/Cy5®. Ex: 496nm, Em: 670nm
  • アプリケーション
    適用あり: Flow Cyt, IPmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • エピトープ
    This antibody recognises an epitope on the 25kDa epsilon chain of the CD3/TCR complex.
  • 特記事項
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.


    In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining and is a good immunoprecipitating antibody. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, 0.20% Gelatin, PBS and stabilising agent.
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 一次抗体 備考
    In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining and is a good immunoprecipitating antibody. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).
  • ポリ/モノ
    モノクローナル
  • クローン名
    C363.29B
  • アイソタイプ
    IgG2c
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能
    The CD3 complex mediates signal transduction.
  • 配列類似性
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

画像

  • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (PE/Cy5) ab25531 (1 µg/106cells) and Rat Anti-Mouse CD19-PE.

参考文献

This product has been referenced in:
  • Carrithers MD  et al. Differential adhesion molecule requirements for immune surveillance and inflammatory recruitment. Brain 123 ( Pt 6):1092-101 (2000). Read more (PubMed: 10825349) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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