Anti-CD3 epsilon 抗体 [145-2C11] (Phycoerythrin) (ab25665)

製品の概要

  • 製品名Anti-CD3 epsilon antibody [145-2C11] (Phycoerythrin)
    CD3 epsilon 一次抗体 製品一覧
  • 製品の詳細
    Armenian Hamster monoclonal [145-2C11] to CD3 epsilon (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性This antibody recognises CD3 epsilon.
  • アプリケーション適用あり: Flow Cyt, WB, IP, Functional Studiesmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue/ cell preparation: H2Kb specific mouse cytotoxic T lymphocyte clone BM10-37.

  • エピトープThis antibody recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
  • 特記事項


    In the presence of Fc receptor bearing accessory cells, this antibody, in the soluble form, can activate primed and naïve T cell in vitro. This antibody also induces redirected lysis of specific target cells by CTL clones, and can block lysis of specific target cells by antigen specific CTL's. Immobilized ab25665 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab25665 may result in apoptotic cell death.

製品の特性

  • 製品の状態Liquid
  • 保存方法Store at +4°C in the dark. Do not freeze.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • 精製度IgG fraction
  • 一次抗体 備考In the presence of Fc receptor bearing accessory cells, this antibody, in the soluble form, can activate primed and naïve T cell in vitro. This antibody also induces redirected lysis of specific target cells by CTL clones, and can block lysis of specific target cells by antigen specific CTL's. Immobilized ab25665 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab25665 may result in apoptotic cell death.
  • ポリ/モノモノクローナル
  • クローン名145-2C11
  • アイソタイプIgG
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
WB
IP
Functional Studies
  • 追加情報Flow Cyt: Use 1µg for 106 cells.
    FuncS: Use at an assay dependent dilution. Ab25665 can be used for in vitro depletion of CD3 positive cells and for in vitro activation of T cells.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能The CD3 complex mediates signal transduction.
    • 配列類似性Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD3 epsilon antibody
      • CD3E antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Anti-CD3 epsilon antibody [145-2C11] (Phycoerythrin) 画像

    • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [145-2C11] (Phycoerythrin) ab25665 (1 µg/106 cells), and Rat Anti-Mouse CD19-FITC.

    Anti-CD3 epsilon antibody [145-2C11] (Phycoerythrin) (ab25665) 使用論文

    ab25665 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab25665.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"