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Tissue/ cell preparation: H2Kb specific mouse cytotoxic T lymphocyte clone BM10-37.
In the presence of Fc receptor bearing accessory cells, this antibody, in the soluble form, can activate primed and naïve T cell in vitro. This antibody also induces redirected lysis of specific target cells by CTL clones, and can block lysis of specific target cells by antigen specific CTL's. Immobilized ab25665 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab25665 may result in apoptotic cell death.
Our Abpromise guarantee covers the use of ab25665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [145-2C11] (Phycoerythrin) ab25665 (1 µg/106 cells), and Rat Anti-Mouse CD19-FITC.
ab25665 has not yet been referenced specifically in any publications.