The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報1. Counting and identification of mature T lymphocytes in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
2. In combination with other monoclonal antibodies, this antibody may also be useful for thediagnosis of T cell leukaemias and lymphomas.
CD3 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD3 (PE) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: 1.0
機能The CD3 complex mediates signal transduction.
関連疾患Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.