製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/100. PubMed: 18628996
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 23 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The recommended starting incubation time is 10min.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20126467
ICC/IF Use at an assay dependent concentration. PubMed: 23874197

ターゲット情報

  • 機能The CD3 complex mediates signal transduction.
  • 関連疾患Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • 配列類似性Contains 1 ITAM domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

Anti-CD3 antibody 画像

  • ab5690 at 1/100 staining mouse lymph node tissue sections by IHC-Fr. The tissue was acetone fixed and blocked with serum before incubation with the antibody for 1 hour. An Alexa Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.

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  • Anti-CD3 antibody (ab5690) + Jurkat Cell Lysate

    Predicted band size : 23 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • No positive immunostaining for CD3ε, a pan T cell marker (ab5690), was detected in the corneas of scrambled siRNA-treated mice (A) at 5 days p.i. In contrast, positive immunostaining (red) was observed in the peripheral cornea of HIF-1α silenced animals (B). The control sections shown in (C) and (D) were immunostained with species-specific IgG and were positive for SYTOX Green nuclear stain only. Images shown are representative of three independent experiments each with three mice per group. Magnification = 180×; inset = 335×.

  • ab5690 staining rat spleen tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in sodium citrate buffer (10mM Sodium Citrate, 0.05% Tween 20, pH 6.0) prior to blocking in 5% normal goat serum + 1% BSA for 2 hours at 37°C.  The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4°C.  A biotinylated goat anti-rabbit antibody, diluted 1/100, was used as the secondary.

    See Abreview

  • ab5690 staining CD3 in the lymphatic nodule of rat spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 1% BSA for 25 minutes at 20°C followed by incubation with the primary antibody at a 1/100 dilution for 1 hour at 20°C. A biotin-conjugated goat anti-rat polyclonal was used undiluted as the secondary antibody.
    CD3 positive cells are mainly distributed in PALS (T-cell region), while sporadic CD3 positive cells are identified in lymphoid follicle and germinal center (B-cell region).

    See Abreview

  • ab5690 staining CD3 in human lymph node tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citric buffer pH 6.0, prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

Anti-CD3 antibody (ab5690) 使用論文

This product has been referenced in:
  • Xiao LI  et al. Multiple myeloma-associated skin light chain amyloidosis: A case of misdiagnosis. Oncol Lett 11:3617-3620 (2016). IHC ; Human . Read more (PubMed: 27284363) »
  • Qi X  et al. Development of inCVAX, In situ Cancer Vaccine, and Its Immune Response in Mice with Hepatocellular Cancer. J Clin Cell Immunol 7:N/A (2016). IHC-P ; Mouse . Read more (PubMed: 27656328) »

See all 71 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rhesus monkey Tissue sections (Brain Tissue)
Specification Brain Tissue
Username

Eli Shobin

Verified customer

投稿 Dec 07 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 26 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 24°C
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Permeabilization Yes - Triton X-100 (0.3%)
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 10 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step DakoCytomation x0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Permeabilization No
Fixative Formaldehyde
Username

Hongwei Shao

Verified customer

投稿 Jan 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Normal horse blocking serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step Other
Sample Rat Tissue sections (Alveolar bone)
Specification Alveolar bone
Permeabilization No
Fixative Formaldehyde
Username

Ms. Dong-Eun Lee

Verified customer

投稿 Oct 15 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris HCl ph10
Sample Mouse Tissue sections (Synovium)
Specification Synovium
Permeabilization No
Fixative zinc buffered formalin
Username

Abcam user community

Verified customer

投稿 Aug 08 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 05 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Antibody Block as blocking agent for 10 minute(s) · Concentration: 100%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1 Mild
Sample Human Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative 10% NBF
Username

Abcam user community

Verified customer

投稿 Dec 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample Rat Tissue sections (spleen)
Specification spleen
Blocking step solution from Dako as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. R Berahovich

Verified customer

投稿 Aug 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 100C 15min in 10 mM Sodium Citrate Buffer (pH 6.0), cooled for 30min, then used 3% H2O2 for 10min
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 23 2013

1-10 of 67 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"