製品の概要

  • 製品名Anti-CD2BP2 antibody
    CD2BP2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to CD2BP2
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    PKRKVTFQGVGDE

    , corresponding to N terminal amino acids 2-14 of Human CD2BP2.

  • ポジティブ・コントロール
    • W: Human lymph node, thymus and tonsil lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide.
    Constituents: 0.5% BSA, 0.5mg/ml Tris saline. pH 7.3.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab32899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/32000.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).

ターゲット情報

Anti-CD2BP2 antibody 画像

  • ab32899 (5µg/ml) shows staining of CD2BP2 in paraffin embedded Human Spleen following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows nucleolar staining in select splenocytes.

  • Anti-CD2BP2 antibody (ab32899) at 0.3 µg/ml + 35ug of human thymus lysate in RIPA buffer

    Predicted band size : 38 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour.
    Detected by chemiluminescence.

Anti-CD2BP2 antibody (ab32899) 使用論文

ab32899 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab32899.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"