Anti-CD28 抗体 [CD28.2] - Low endotoxin, Azide free (ab85986)
Key features and details
- Mouse monoclonal [CD28.2] to CD28 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free
CD28 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CD28.2] to CD28 - Low endotoxin, Azide free -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Non human primates -
免疫原
Tissue, cells or virus corresponding to CD28. Human CD28 transfected murine T cell hybridoma.
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ポジティブ・コントロール
- Flow Cyt: CD28 positive lymphocytes.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.20
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
Azide free. -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. Endotoxin level is less than or equal to 0.01 EU/ug, as determined by the LaL assay. -
ポリ/モノ
モノクローナル -
クローン名
CD28.2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
- PE Anti-CD28 antibody [CD28.2], prediluted (ab134418)
- FITC Anti-CD28 antibody [CD28.2] (ab134472)
- PerCP/Cy5.5® Anti-CD28 antibody [CD28.2], prediluted (ab157318)
- PE/Cy7® Anti-CD28 antibody [CD28.2] (ab234276)
- Biotin Anti-CD28 antibody [CD28.2] (ab267385)
- APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)
- PerCP Anti-CD28 antibody [CD28.2] (ab88034)
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85986の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use a concentration of 1 - 12 µg/ml.
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特記事項 |
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Flow Cyt
Use a concentration of 1 - 12 µg/ml. |
ターゲット情報
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機能
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
組織特異性
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 940 Human
- Omim: 186760 Human
- SwissProt: P10747 Human
- Unigene: 591629 Human
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別名
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
画像
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Flow cytometry surface staining pattern of human peripheral blood stained using ab85986 GAM APC.
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Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab85986 GAM APC.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab85986 は 11 報の論文で使用されています。
- Liu R et al. Serum-derived exosomes containing NEAT1 promote the occurrence of rheumatoid arthritis through regulation of miR-144-3p/ROCK2 axis. Ther Adv Chronic Dis 12:2040622321991705 (2021). PubMed: 33995991
- Fehrholz M et al. Impact of the New Generation Reconstituted Surfactant CHF5633 on Human CD4+ Lymphocytes. PLoS One 11:e0153578 (2016). PubMed: 27077658
- Urso K et al. NFATc3 regulates the transcription of genes involved in T-cell activation and angiogenesis. Blood 118:795-803 (2011). PubMed: 21642596
- Chen ML et al. Antipsychotic drugs suppress the AKT/NF-?B pathway and regulate the differentiation of T-cell subsets. Immunol Lett 140:81-91 (2011). Functional Studies ; Human . PubMed: 21763349
- Scharschmidt E et al. Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol 24:3860-73 (2004). PubMed: 15082780