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CHO cell line CN5.1 transfected with a murine cDNA (J. Immunology; 149 (2); 380; PubMed:1320641)
Our Abpromise guarantee covers the use of ab95559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5µg for 105 cells. Use at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.|
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