製品の概要

  • 製品名Anti-CD276 antibody [MM0104-20J12]
    CD276 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MM0104-20J12] to CD276
  • アプリケーション適用あり: Flow Cyt, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to the extracellular domain of Human CD276

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 58 kDa.

ターゲット情報

  • 機能May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
  • 組織特異性Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
  • 配列類似性Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4Ig B7 H3 antibody
    • 4Ig-B7-H3 antibody
    • AU016588 antibody
    • B7 H3 antibody
    • B7 homolog 3 antibody
    • B7-H3 antibody
    • B7H3 antibody
    • B7RP-2 antibody
    • CD_antigen=CD276 antibody
    • CD276 antibody
    • CD276 antigen antibody
    • CD276 molecule antibody
    • CD276_HUMAN antibody
    • Costimulatory molecule antibody
    • Flags: Precursor antibody
    • PSEC0249 antibody
    • UNQ309/PRO352 antibody
    see all

Anti-CD276 antibody [MM0104-20J12] 画像

  • IHC image of ab89133 staining in Human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89133, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing THP1 cells stained with ab89133 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89133, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-CD276 antibody [MM0104-20J12] (ab89133) 使用論文

ab89133 has not yet been referenced specifically in any publications.

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