Anti-CD27 抗体 - Azide free (ab10456)

製品の概要

  • 製品名Anti-CD27 antibody - Azide free
    CD27 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to CD27 - Azide free
  • 特異性This antibody does not cross react with recombinant human CD30, recombinant human NGF R, recombinant human soluble TNF RI, and recombinant human soluble TNF RII.
  • アプリケーション適用あり: ELISA, Flow Cyt, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human CD27 extracellular domain expressed in Sf2I insect cells.

  • ポジティブ・コントロール
  • 特記事項Prior to reconstitution, store at -20 °C. Reconstituted product may be stored at 2-8 °C for at least one month. For prolonged storage, freeze in working aliquots at -20°C.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
Flow Cyt
WB
  • 追加情報ELISA: Use at a concentration of 0.5 - 1 µg/ml in indirect ELISA (determined to detect a limit of ~0.06 ng/well of human CD27).
    Flow Cyt: Use 0.5µg for 106 cells in indirect flow cytometry (determined using the appropriate secondary reagent for indirect immunofluorescence staining of T-lymphocytes).
    WB: Use at a concentration of 0.1 - 0.2 µg/ml (determined using human CD27 at 5ng/lane and 20ng/lane under non-reducing and reducing conditions, respectively). Predicted molecular weight: 31 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
    • 組織特異性Found in most T-lymphocytes.
    • 配列類似性Contains 3 TNFR-Cys repeats.
    • 翻訳後修飾Phosphorylated and O-glycosylated.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD 27 antibody
      • CD27 antibody
      • CD27 antigen antibody
      • CD27 molecule antibody
      • CD27_HUMAN antibody
      • CD27L receptor antibody
      • LPFS2 antibody
      • MGC20393 antibody
      • OTTHUMP00000238557 antibody
      • S152 antibody
      • T cell activation antigen CD27 antibody
      • T cell antivation antigen S152 antibody
      • T-cell activation antigen CD27 antibody
      • T14 antibody
      • TNFRSF 7 antibody
      • TNFRSF7 antibody
      • TNFSF7 antibody
      • Tp 55 antibody
      • Tp55 antibody
      • Tumor necrosis factor receptor superfamily member 7 antibody
      see all

    Anti-CD27 antibody - Azide free (ab10456) 使用論文

    ab10456 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (Leukaemia cell lines)
    Loading amount 10 µg
    Specification Leukaemia cell lines
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in PBS-
    Username

    Dr. Mark Holland

    Verified customer

    投稿 Mar 30 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"