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Our Abpromise guarantee covers the use of ab89896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/20 - 1/200.|
|Neutralising||Use at an assay dependent concentration. Proliferation of human PBMCs elicited by TNFSF4 (25ng/mL) can be neutralized by increasing concentrations of ab89896 in a dose-dependent manner. The ED50 is between 40-80ng/mL.|
|Flow Cyt||1/50 - 1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ab89896 staining CD252 in Cynomolgus Monkey lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1/25) for 30 minutes at room temperture. An undiluted Biotin-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
ab89896 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"