Anti-CD252 抗体 [MM0505-8S23] (ab89896)

Abreviews (1)Q&A (7)
Customer reviews and FAQs

製品の概要

  • 製品名Anti-CD252 antibody [MM0505-8S23]
    CD252 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MM0505-8S23] to CD252
  • アプリケーション適用あり: IHC-P, IHC-Fr, Neutralising, Flow Cytmore details
  • 種交差性
    交差種: Human, Cynomolgus monkey
  • 免疫原

    Recombinant full length Human CD252

  • 特記事項The antibody is specific to OX40L.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/20 - 1/200.
Neutralising Use at an assay dependent concentration. Proliferation of human PBMCs elicited by TNFSF4 (25ng/mL) can be neutralized by increasing concentrations of ab89896 in a dose-dependent manner. The ED50 is between 40-80ng/mL.
Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
  • 関連疾患Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance.
  • 配列類似性Belongs to the tumor necrosis factor family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • CD 134L antibody
      • CD 252 antibody
      • CD134 antibody
      • CD134 ligand antibody
      • CD134L antibody
      • CD252 antibody
      • CD252 antigen antibody
      • glycoprotein 34 kd antibody
      • Glycoprotein Gp34 antibody
      • GP34 antibody
      • OX-40L antibody
      • OX40 antigen ligand antibody
      • OX40 ligand antibody
      • OX40L antibody
      • TAX transcriptionally-activated glycoprotein 1 antibody
      • TNFL4_HUMAN antibody
      • Tnfsf4 antibody
      • Tumor necrosis factor (ligand) superfamily member 4 antibody
      • Tumor necrosis factor ligand superfamily member 4 antibody
      • TXGP1 antibody
      see all

    Anti-CD252 antibody [MM0505-8S23] 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD252 antibody [MM0505-8S23] (ab89896)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD252 antibody [MM0505-8S23] (ab89896)This image is courtesy of an Abreview submitted by Jing Ma

      ab89896 staining CD252 in Cynomolgus Monkey lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1/25) for 30 minutes at room temperture. An undiluted Biotin-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    Anti-CD252 antibody [MM0505-8S23] (ab89896) 使用論文

    ab89896 has not yet been referenced specifically in any publications.

    ab89896 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab89896 の abreview を投稿する ab89896 に関する問い合わせをする

    8 Abreviews or Q&A

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD252 antibody [MM0505-8S23]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rt°C
    Antigen retrieval step Enzymatic
    Sample Cynomolgus Monkey Tissue sections (Lymph node)
    Specification Lymph node
    Permeabilization No
    Fixative Formaldehyde
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    abreview image
    Username

    Jing Ma

    Verified customer

    投稿 Aug 30 2013

    Hi,

    You recommend your anti-OX40L (ab89896) for IHC/IHC-Fr.. Do you have any evidence/images to demonstrate this?

    Cheers,

    Thank you very much for your interest in ab89896.

    This clone was tested using frozen human inflammatory lymph nodes by immunofluorescence, showing positive subgroup cells.


    We do not currently have an image of ab89896 in IHC-Fr...

    Read More

    Customer kindly contacted us regarding any additional data we have on the neutralizing properties of ab89896 against PBMC proliferation. The customer is specifically looking for any endotoxin levels that were measured.

    For each lot of ab89896, the level of LPS is <0.01EU/ug IgG.

    Thank you very much for your continued help.

    The customer has some additional questions regarding the product:
    1. Did Abcam use fresh or thawed PBMC?
    2. How many PBMC were culture in each well?, what media did Abcam use? Did they used ...

    Read More

    Thank you for your email.

    Here are some answers:

    1. Did Abcam use fresh or thawed PBMC?

    We used freshed ones!

    2. How many PBMC were culture in each well?, what media did Abcam use? Did they
    used activation apart ...

    Read More

    Regarding ab89896:

    Do you have any records to the Endotoxin level?

    The datasheet states there are no preservatives to the antibody, is it azid free?
    What protocol was used in order to demonstrate the antibody neutralization activi...

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    Thank you for contacting us.

    The endotoxin level is <0.5EU/mg antibody.

    The antibody is azide free.

    Customer needs optimizing the conditions on his/her own as the culture condition wont be the same. The assay was performed...

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    What protocol was used to test this antibody in Flow Cytometry?

    Thank you for patience while I contacted the lab.
    Unfortunately we do not have a detailed protocol for this antibody
    In essence they use the primary antibody at2.5ug per1 x 10(6) cells (human PBMCs stimulated with cytokines) and then follow a...

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    Has the endotoxin levels been measured for this antibody?

    Thank you for your interest in ab89896. I have confirmed with the laboratory that the endotoxin level is <0.5EU/mg antibody. I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    On ds: Neut of TNFSF4 bioactivity? protcol?Which test system it was used in? Was it tested with the anti-CD134 antibody cross-linked with a secondary antibody?

    Human recombinant TNFSF4 (with Poly-His taq) can stimulate the proliferation of human PBMCs, in the presence of cross-linking antibody and PHA (phytohemagglutinin). Under this condition, proliferation of human PBMCs elicited by TNFSF4 (25ng/mL) can...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"