Anti-CD244 抗体 (ab113289)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CD244
  • 特異性
    At least three isoforms are known to exist. ab113289 is predicted to not cross-react with other family members.
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    16 amino acid peptide near the C terminus of Human CD244.

  • ポジティブ・コントロール
    • Daudi cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 20 µg/ml.

ターゲット情報

  • 機能
    Modulate other receptor-ligand interactions to enhance leukocyte activation.
  • 組織特異性
    Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils.
  • 配列類似性
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2B4 antibody
    • C9.1 antibody
    • CD244 antibody
    • CD244 antigen antibody
    • CD244 molecule antibody
    • CD244 molecule natural killer cell receptor 2B4 antibody
    • CD244 natural killer cell receptor 2B4 antibody
    • CD244_HUMAN antibody
    • F730046O15Rik antibody
    • h2B4 antibody
    • Ly90 antibody
    • NAIL antibody
    • Natural killer cell activation-inducing ligand antibody
    • Natural killer cell receptor 2B4 antibody
    • NK cell activation inducing ligand antibody
    • NK cell activation inducing ligand NAIL antibody
    • NK cell activation-inducing ligand antibody
    • NK cell type I receptor protein 2B4 antibody
    • NKR2B4 antibody
    • Nmrk antibody
    • Non-MHC restricted killing associated antibody
    • OTTHUMP00000027884 antibody
    • p38 antibody
    • signaling lymphocytic activation molecule 4 antibody
    • SLAM family, member 4 antibody
    • SLAMF4 antibody
    see all

画像

  • Lane 1 : Anti-CD244 antibody (ab113289) at 1 µg/ml
    Lane 2 : Anti-CD244 antibody (ab113289) at 2 µg/ml

    Lane 1 : Daudi cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 42 kDa
  • Immunofluorescence analysis of Daudi cells, staining CD244 with ab113289 at 20 µg/ml.

参考文献

ab113289 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH 6.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
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投稿 Jan 27 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

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Verified customer

投稿 Mar 01 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (spleen)
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

投稿 Feb 01 2016


According to the SwissProt link below, CD244 has 4 isoforms that are 42, 41, 37, and 31 kDa. Thus, it appears the band in the image you referenced likely corresponds to the 31 kDa isoform 4.
http://www.uniprot.org/uniprot/Q9BZW8

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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