製品の概要

  • 製品名
    Anti-CD24 antibody [M1/69]
    CD24 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [M1/69] to CD24
  • アプリケーション
    適用あり: WB, IHC-Fr, Flow Cyt, IHC-P, Functional Studies, ICC/IFmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes.

  • ポジティブ・コントロール
    • C57BL/6 mouse splenocytes.

アプリケーション

Our Abpromise guarantee covers the use of ab64064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-Fr
Flow Cyt
IHC-P
Functional Studies
ICC/IF
  • 追加情報
    Flow Cyt: Use 0.25µg for 106 cells in 100µl volume.
    FuncS: Use at an assay dependent dilution. in vitro induction of thymocyte maturation2 and complement-mediated cytotoxicity.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution. Use zinc-fixed paraffin-embedded sections or formalin-fixed paraffin-embedded sections.
    IHC-Fr: Use at an assay dependent dilution. Use acetone-fixed frozen sections.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 9 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
    • 組織特異性
      B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
    • 関連疾患
      Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
    • 配列類似性
      Belongs to the CD24 family.
    • 翻訳後修飾
      Extensively O-glycosylated.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD 24 antibody
      • CD24 antibody
      • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
      • CD24 antigen antibody
      • CD24 molecule antibody
      • CD24_HUMAN antibody
      • CD24A antibody
      • FLJ22950 antibody
      • FLJ43543 antibody
      • GPI linked surface mucin antibody
      • Heat stable antigen antibody
      • HSA antibody
      • MGC75043 antibody
      • Nectadrin antibody
      • Signal transducer CD24 antibody
      • Small cell lung carcinoma cluster 4 antigen antibody
      see all

    Anti-CD24 antibody [M1/69] 画像

    • Flow cytometry of C57BL/6 mouse splenocytes stained with ab64064, at 0.25 µg per 106 cells, followed by anti-rat IgG FITC.
    • Immunohistochemical analysis of Mouse spleen tissue, staining Perforin with ab64064.

      Tissue was fixed with paraformaldehyde and permeabilized with Tween-20; antigen retrieval was by heat mediation in Tris-EDTA buffer. Samples were incubated with primary antibody (1/50 in diluent) for 30 minutes at 20°C. An undiluted Histofine®-conjugated goat anti-rat polyclonal IgG was used as the secondary antibody.

      See Abreview

    Anti-CD24 antibody [M1/69] (ab64064) 使用論文

    This product has been referenced in:
    • Cuevas E  et al. Lin41/Trim71 is essential for mouse development and specifically expressed in postnatal ependymal cells of the brain. Front Cell Dev Biol 3:20 (2015). Read more (PubMed: 25883935) »
    • Neirinckx V  et al. Adult Bone Marrow Neural Crest Stem Cells and Mesenchymal Stem Cells Are Not Able to Replace Lost Neurons in Acute MPTP-Lesioned Mice. PLoS One 8:e64723 (2013). IHC-Fr, ICC/IF ; Mouse . Read more (PubMed: 23741377) »

    See all 2 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T, K562 cell)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    15 µg
    Specification
    293T, K562 cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 May 18 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney Progemitor Cells)
    Permeabilization
    No
    Specification
    Kidney Progemitor Cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Maria Pearce

    Verified customer

    投稿 Aug 01 2016

    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A/G
    Sample
    Human Cell lysate - whole cell (MDA-MB-231)
    Specification
    MDA-MB-231
    Total protein in input
    30 µg
    Username

    Abcam user community

    Verified customer

    投稿 May 01 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
    Sample
    Human Tissue sections (human colon)
    Specification
    human colon
    Permeabilization
    Yes - tween-20
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 01 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample
    Human Cell (MCF7)
    Specification
    MCF7
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 01 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Specification
    Spleen
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
    Permeabilization
    Yes - Wash-Buffer from Dako with Tween
    Username

    Mr. Rudolf Jung

    Verified customer

    投稿 Oct 01 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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