製品の概要

  • 製品名Anti-CD22 (phospho Y822) antibody [Y506]
    CD22 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y506] to CD22 (phospho Y822)
  • 特異性ab32123 recognises CD22 phosphorylation on Tyrosine 822. This antibody is predicted to detect splice isoform 2 based on sequence homology.
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: IHC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 822 of human CD22

  • ポジティブ・コントロール
    • Raji cell lysate and K562 cells
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 95 kDa).
Flow Cyt 1/10. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for IHC or IP.
  • ターゲット情報

    • 機能Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
    • 組織特異性B-lymphocytes.
    • 配列類似性Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • ドメインContains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • 翻訳後修飾Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
      Phosphorylated on tyrosine residues by LYN.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • B cell receptor CD22 precursor antibody
      • B lymphocyte cell adhesion molecule antibody
      • B-cell receptor CD22 antibody
      • B-lymphocyte cell adhesion molecule antibody
      • BL CAM antibody
      • BL-CAM antibody
      • BLCAM antibody
      • CD 22 antibody
      • CD22 antibody
      • CD22 antigen antibody
      • CD22 molecule antibody
      • CD22 protein antibody
      • CD22_HUMAN antibody
      • Lectin 2 antibody
      • Leu14 antibody
      • Lyb8 antibody
      • MGC130020 antibody
      • sialic acid binding Ig like lectin 2 antibody
      • Sialic acid binding immunoglobulin like lectin 2 antibody
      • Sialic acid-binding Ig-like lectin 2 antibody
      • SIGLEC 2 antibody
      • Siglec-2 antibody
      • SIGLEC2 antibody
      • T cell surface antigen Leu 14 antibody
      • T-cell surface antigen Leu-14 antibody
      see all

    Anti-CD22 (phospho Y822) antibody [Y506] 画像

    • All lanes : Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution

      Lane 1 : Untreated Raji cell lysate
      Lane 2 : Raji cell lysate treated with Pervanadate


      Predicted band size : 95 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized K562 cells, untreated (green) or Pervanadate treated (red) using ab32123

    Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) 使用論文

    This product has been referenced in:
    • Séïté JF  et al. IVIg modulates BCR signaling through CD22 and promotes apoptosis in mature human B lymphocytes. Blood 116:1698-704 (2010). Flow Cyt ; Human . Read more (PubMed: 20516366) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (Splenic B cells)
    Permeabilization Yes - BD Perm buffer III
    Gating Strategy Single cells, B220+ B cells
    Specification Splenic B cells
    Preparation Cell harvesting/tissue preparation method: Splenocyte isolation from fresh spleens followed by RBC lysis
    Sample buffer: Pervanadate stimulation
    Fixation Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Dec 02 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"