Anti-CD200R 抗体 [OX102] (Phycoerythrin) (ab34135)

製品の概要

  • 製品名
    Anti-CD200R antibody [OX102] (Phycoerythrin)
    CD200R 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [OX102] to CD200R (Phycoerythrin)
  • 標識
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性
    This antibody is specific for the CD200R ligand.
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Tissue/ cell preparation: Membrane fraction of thioglycollate elicited rat peripheral cells.

  • 特記事項


    This antibody has been shown to block the interaction of OX2 ligand with CD200.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab34135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/1 - 1/10.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.

ターゲット情報

  • 機能
    Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
  • 組織特異性
    Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
  • 配列類似性
    Belongs to the CD200R family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾
    The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 200R antibody
    • CD200 cell surface glycoprotein receptor antibody
    • CD200 receptor 1 antibody
    • Cd200r1 antibody
    • Cell surface glycoprotein CD200 receptor 1 antibody
    • Cell surface glycoprotein OX2 receptor 1 antibody
    • Cell surface glycoprotein OX2 receptor antibody
    • cell surface glycoprotein receptor CD200 antibody
    • CRTR 2 antibody
    • CRTR2 antibody
    • HCRTR2 antibody
    • MO2R1_HUMAN antibody
    • MOX2 receptor antibody
    • MOX2R antibody
    • OX2R antibody
    see all

画像

  • Overlay histogram showing Rat blood cells stained with ab34135 (red line). The sample was prepared by lysing Red Blood cells and cells were fixed with formaldehyde. The cells were then incubated with the antibody for 1 hour diluted 1/100 in PBS/1% BSA/0.01% Azide. The cell population was gated on monocytes.

    See Abreview

プロトコール

参考文献

ab34135 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Monkey Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after lysis of red blood cells
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
on monocytes
Username

Abcam user community

Verified customer

投稿 Mar 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (from blood)
Specification
from blood
Preparation
Cell harvesting/tissue preparation method: after lysis of red blood cells
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
on monocytes
Username

Abcam user community

Verified customer

投稿 Mar 23 2011

Application
Flow Cytometry
Sample
Mouse Cell (from blood)
Specification
from blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
on monocytes
Username

Abcam user community

Verified customer

投稿 Mar 23 2011

Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: from buffy coat
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Permeabilization
No
Gating Strategy
monocytes
Username

Abcam user community

Verified customer

投稿 Mar 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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