Anti-CD19 抗体 [MB19-1] (Allophycocyanin) (ab25510)

製品の概要

  • 製品名Anti-CD19 antibody [MB19-1] (Allophycocyanin)
    CD19 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MB19-1] to CD19 (Allophycocyanin)
  • 標識Allophycocyanin. Ex: 645nm, Em: 660nm
  • 特異性ab25510 recognises CD19 B cell differentiation antigen
  • アプリケーション適用あり: Flow Cyt, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    CD19+ mouse pre-B cell line, 300.19

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.2µg for 106 cells. ab37353-Mouse monoclonal IgA, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • 関連疾患Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 配列類似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻訳後修飾Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

Anti-CD19 antibody [MB19-1] (Allophycocyanin) 画像

  • ab25510 used in Flow Cytometry.
    Red: ab25510 at a 1/100 dilution staining CD19 in murine lymph cells. Cells were mechanically dissociated from the lymph node and analysed live.
    Black: Isotype control.

    See Abreview

Anti-CD19 antibody [MB19-1] (Allophycocyanin) (ab25510) 使用論文

This product has been referenced in:
  • Sato S  et al. CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity. J Immunol 157:4371-8 (1996). Read more (PubMed: 8906812) »
  • Krop I  et al. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol 26:238-42 (1996). Read more (PubMed: 8566073) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rabbit Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

投稿 Dec 21 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Pig Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

投稿 Dec 21 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Apr 25 2012

Application Flow Cytometry
Sample Rat Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Apr 13 2012

My recommendations are as follows:

Anti-Mouse IgG1 secondary, pre-adsorbed, FITC-conjugate - ab98692
Anti-Mouse IgG1 secondary, pre-adsorbed, APC-conjugate - We unfortunately do not have an APC conjugate.
Mouse IgG1 isotype control...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: from buffy coat
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

投稿 Nov 15 2011

Application Flow Cytometry
Sample Dog Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

投稿 Aug 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (cells from lymph node)
Specification cells from lymph node
Preparation Cell harvesting/tissue preparation method: mechanical dissociation of the lymph node
Sample buffer: PBS/BSA 0.1%
Permeabilization No
Gating Strategy on alive (PI-) cells
Username

Abcam user community

Verified customer

投稿 Jun 28 2011

I have discussed with my colleagues the high background you are observing with the blocking peptide and we are very puzzled at those results. If the antibody did not work we would expect no staining or high staining and to us the antibody works very we...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"