製品の概要

  • 製品名
    Anti-CD19 antibody [6D5]
    CD19 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [6D5] to CD19
  • 特異性
    CD19 B-cell differentiation antigen
  • アプリケーション
    適用あり: Flow Cyt, IP, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Mouse CD19-expressing K562 human erythroleukemia cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • 関連疾患
    Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 配列類似性
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻訳後修飾
    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

画像

  • Immunohistochemical analysis of renal lymph node with PC3-tumour metastases, staining CD19 with ab25232.

    Cryosections were stored at -80°C and incubated with primary antibodies for 1 hour. After washing with PBS, sections were stained for 1 hour with secondary antibodies.
  • Flow Cytometry analysis of BALB/c mouse splenocyte cells labeling CD19 with ab25232 at 1 μg/106 cells dilution, followed by Rat Anti-Mouse CD3ε-FITC then by Mouse Anti-Rat IgG2a-PE.

参考文献

This product has been referenced in:
  • Di Lullo AM  et al. An "ex vivo model" contributing to the diagnosis and evaluation of new drugs in cystic fibrosis. Acta Otorhinolaryngol Ital 37:207-213 (2017). ICC ; Human . Read more (PubMed: 27897275) »
  • Sawyer DM  et al. Lymphocytes influence intracranial aneurysm formation and rupture: role of extracellular matrix remodeling and phenotypic modulation of vascular smooth muscle cells. J Neuroinflammation 13:185 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27416931) »

See all 11 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Ileal - Peyer's Patch)
Permeabilization
No
Specification
Ileal - Peyer's Patch
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Username

Jack Robertson

Verified customer

投稿 Oct 04 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 5 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 17 2015

We have one unconjugated antibody ab25232 which unfortunately hasn't been tested in functional assays though it has similar clone number then the tested conjugated ones.

In case you are interested in buying and trying this antibody then I wou...

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Thank you for contacting us.

Please check the following antibodies;


http://www.abcam.com/cd19-antibody-6d5-pe-cy5-5-ab25548.html

http://www.abcam.com/cd19-antibody-mb19-1-pe-cy5-5-ab25384.html

I hope this inform...

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Thank you very much for your interest in ab25232.
To our knowledge, ab25232 has not been tested in IHC-P yet.
We do offer the following antibody that is tested and guaranteed for mouse and IHC-P and might be suitable for your purpose of ident...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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