製品の概要

  • 製品名Anti-CD19 antibody [2E2B6B10]
    CD19 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2E2B6B10] to CD19
  • アプリケーション適用あり: Flow Cyt, ICC/IF, WB, IHC-P, IHC-Fr, ELISAmore details
  • 種交差性
    交差種: Human, Recombinant Fragment
  • 免疫原

    Ni-NTA purified recombinant human CD19 expressed in E. Coli strain M15.

  • ポジティブ・コントロール
    • Recombinant human CD19 and normal human lymph node tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 10µl for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
WB 1/2000 - 1/4000. Detects a band of approximately 20 kDa (predicted molecular weight: 61 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/200 - 1/400.
ELISA 1/10000.

ターゲット情報

  • 機能Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • 関連疾患Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 配列類似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻訳後修飾Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

Anti-CD19 antibody [2E2B6B10] 画像

  • Immnohistochemical staining of paraffin embedded normal human, adult lymph node tissue, using ab31947. Showing cytoplasmic staining. Secondary staining was carried out using an HRP conjugated antibody before colour development using DAB.
  • Overlay histogram showing peripheral blood lymphocytes stained with ab31947 (red line). The cells were incubated with the antibody (ab31947, 10µl/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

Anti-CD19 antibody [2E2B6B10] (ab31947) 使用論文

This product has been referenced in:
  • Bourgine PE  et al. Osteoinductivity of engineered cartilaginous templates devitalized by inducible apoptosis. Proc Natl Acad Sci U S A 111:17426-31 (2014). IHC-P ; Human . Read more (PubMed: 25422415) »
  • Sohal SS  et al. Evaluation of epithelial mesenchymal transition in patients with chronic obstructive pulmonary disease. Respir Res 12:130 (2011). Read more (PubMed: 21970519) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Peripheral blood mononuclear cells (PBMCs))
Permeabilization Yes - 0.5% TX100
Specification Peripheral blood mononuclear cells (PBMCs)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

投稿 Nov 04 2016

Abreviews
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Tissue sections (Human blood film)
Specification Human blood film
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Jun 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human peripheral blood mononuclear cells)
Specification Human peripheral blood mononuclear cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Peripheral blood lymphocytes)
Specification Peripheral blood lymphocytes
Preparation Cell harvesting/tissue preparation method: Ficoll/Hypaque density centrifugation. RBC lysis.
Sample buffer: PBS
Permeabilization No
Gating Strategy FSC/SSC lymphocytes and then FSC-A/FSC-H singlets
Username

Dr. Josh Gregorio

Verified customer

投稿 Aug 21 2012

Vielen Dank für Ihre Anfrage.

Leider haben wir nicht die unterschiedlichen Phänotypen dieser Proteine getestet. Da es sich allerdings ja um polyklonale Antikörper gegen das Vollprotein handelt, sollten die meisten Varianten erkannt werden, s...

Read More

Thank you for your enquiry. I can confirm that the storage buffer is made of PBS containing 0.03% azide, therefore we do not recommend its direct use in cell cultures as it is cytotoxic; I would recommend to dialyse the antibody to remove the azide...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Chronic lymphocytic leukaemia cells)
Loading amount 18 µg
Specification Chronic lymphocytic leukaemia cells
Gel Running Conditions Reduced Denaturing (12% Tris-Glycine, 60 minutes, 200 volts constant)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Feb 13 2008

Thank you for your enquiry. We have been informed that ab31947 is stored in PBS (without BSA). Please don't hesitate to contact us again if you have further questions.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Rat brain sections)
Specification Rat brain sections
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Background Sniper- Biocare Medical REF BS966L as blocking agent for 10 minute(s) · Concentration: 000% · Temperature: 25°C
Username

Mr. osama mohsen

Verified customer

投稿 Oct 02 2007

We have many products in catalogue. Please find below few of these

http://www.abcam.com/cd19-antibody-epr5906-ab134114.html

http://www.abcam.com/cd19-antibody-epr5906-ab134114.html

http://www.abcam.com/cd19-antibody-2e2b6b10-...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"