Anti-CD18 (phospho S745) 抗体 [EP1288Y] (ab52920)


  • 製品名Anti-CD18 (phospho S745) antibody [EP1288Y]
    CD18 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1288Y] to CD18 (phospho S745)
  • 特異性This antibody only detects CD18 phosphorylated on Serine 745.
  • アプリケーション適用あり: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phospho-peptide corresponding to residues surrounding Ser745 of human CD18.

  • ポジティブ・コントロール
    • Jurkat cell lysate or human tonsil tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Raw 246.7
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab52920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IP 1/100.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • 機能Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • 関連疾患Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • 配列類似性Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • 翻訳後修飾Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 95 subunit beta antibody
    • Beta 2 (antigen CD18 (p95) antibody
    • CD 18 antibody
    • CD18 antibody
    • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
    • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
    • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
    • Complement component 3 receptor 3 and 4 subunit antibody
    • Complement receptor C3 beta subunit antibody
    • Complement receptor C3 subunit beta antibody
    • Integrin beta 2 antibody
    • Integrin beta chain beta 2 antibody
    • Integrin beta-2 (ITGB2) antibody
    • Integrin beta-2 antibody
    • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
    • ITB2_HUMAN antibody
    • ITGB2 antibody
    • LAD antibody
    • LCAMB antibody
    • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
    • Leukocyte cell adhesion molecule CD18 antibody
    • LFA 1 antibody
    • LFA1 antibody
    • Lymphocyte function associated antigen 1 (LFA1) antibody
    • Lymphocyte function associated antigen 1 antibody
    • MAC 1 antibody
    • MAC1 antibody
    • Macrophage antigen 1 (mac1) beta antibody
    • MF17 antibody
    • MFI7 antibody
    • OTTHUMP00000115278 antibody
    • OTTHUMP00000115279 antibody
    • OTTHUMP00000115280 antibody
    • OTTHUMP00000115281 antibody
    • OTTHUMP00000115282 antibody
    see all

Anti-CD18 (phospho S745) antibody [EP1288Y] 画像

  • ICC/IF image of ab52920 stained Raw 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab52920 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 1/500 dilution

    Lane 1 : Jurkat cell lysate, untreated
    Lane 2 : Jurkat cell lysate, treated with AP.

    Lysates/proteins at 10 µg per lane.

    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 85 kDa
    Observed band size : 85 kDa
  • Ab52920 (1:250) staining human CD18 in human tonsil tissue by immunohistochemistry using paraffin embedded tissue.
  • Human peripheral blood lymphocytes stained with ab52920 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab52920, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) 使用論文

ab52920 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihre Email.

Beide Nummern (1213063 und GR31039-4) beschreiben dasselbe Lot.

Ich kann bestätigen, dass die Konzentration für dieses Lot 1.283 mg/mL ist.

ich hoffe, diese Information ist hilfre...

Read More

Vielen Dank für Ihren Anruf.

Ich freue mich nun die Konzentration von Lot 1213063 bestätigen zu können:

1.283 mg/mL.

Ich hoffe, dies wird Ihnen beim Einstellen der Isotypkontrolle weiterhelfen. Bitte beachte...

Read More

Thank you for contacting us.

I advise not buying ab52920 if you want to stain all CD18. The antibody is raised against a phosphorylated site on CD18, and will only stain CD18 that is phosphorylated at that site, not total CD18.


Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has...

Read More

Thank you for contacting Abcam.

For ab63388 you get 100 µg at 1mg/ml. For ab52920 you get 100ul butthe concentration varies slightly from batch to batch and so if you could let me know the lot number that you currently have, I will be h...

Read More

Thank you for contacting us. ab63388 hasn't been tested in WB so we can’t confirm the suitability of this antibody due to lack of tested data. However this does not mean this cannot be used in WB this only means we have not tested it. If you are ...

Read More

The ab52920 can be used in Western Blot, Immunohistochemistry (paraffin embedded tissue sections, immunoprecipitation and Immunocytochemistry / Immunofluorescence. I hope this will help!

Thank you for contacting us. Could you let us know the phosphorylated amino acid you are interested in? The integrin beta 2 shows phosphorylation at Serine 745, 756 and at Threonine 758, 759 and 760. The antibodies we have are ab52920 and ab63388 I hop...

Read More