Anti-CD18 抗体 [MEM-48] - Low endotoxin, Azide free (ab46700)

製品の概要

  • 製品名Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free
    CD18 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-48] to CD18 - Low endotoxin, Azide free
  • 特異性The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin ß2 subunit; ß2 integrin).
  • アプリケーション適用あり: Flow Cyt, IP, WB, IHC-Fr, Functional Studiesmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Leucocytes of a patient suffering from a LGL-type leukaemia.

  • ポジティブ・コントロール
    • WB: Kg-1a human leukemia cell lysate
  • 特記事項Preserved by filter sterilization

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab46700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Use under non reducing condition. Predicted molecular weight: 85 kDa. Use under non reducing conditions. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. SDS-PAGE (6% separating gel, 4% stacking gel).
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. This antibody is useful for studies of cell-cell and cell-matrix adhesion. It induces high-affinity conformation of LFA-1 complex.

ターゲット情報

  • 機能Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • 関連疾患Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • 配列類似性Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • 翻訳後修飾Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 95 subunit beta antibody
    • Beta 2 (antigen CD18 (p95) antibody
    • CD 18 antibody
    • CD18 antibody
    • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
    • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
    • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
    • Complement component 3 receptor 3 and 4 subunit antibody
    • Complement receptor C3 beta subunit antibody
    • Complement receptor C3 subunit beta antibody
    • Integrin beta 2 antibody
    • Integrin beta chain beta 2 antibody
    • Integrin beta-2 (ITGB2) antibody
    • Integrin beta-2 antibody
    • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
    • ITB2_HUMAN antibody
    • ITGB2 antibody
    • LAD antibody
    • LCAMB antibody
    • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
    • Leukocyte cell adhesion molecule CD18 antibody
    • LFA 1 antibody
    • LFA1 antibody
    • Lymphocyte function associated antigen 1 (LFA1) antibody
    • Lymphocyte function associated antigen 1 antibody
    • MAC 1 antibody
    • MAC1 antibody
    • Macrophage antigen 1 (mac1) beta antibody
    • MF17 antibody
    • MFI7 antibody
    • OTTHUMP00000115278 antibody
    • OTTHUMP00000115279 antibody
    • OTTHUMP00000115280 antibody
    • OTTHUMP00000115281 antibody
    • OTTHUMP00000115282 antibody
    see all

Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free 画像

  • Overlay histogram showing peripheral blood lymphocytes stained with ab46700 (red line). The cells were incubated with the antibody (ab46700, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700) 使用論文

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