製品の概要

  • 製品名
    Anti-CD18 antibody [MEM-48]
    CD18 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-48] to CD18
  • 特異性
    Human CD18 antigen. This antibody recognizes all three leucocyte integrins.
  • アプリケーション
    適用あり: ICC/IF, IHC-P, Flow Cyt, IHC-Fr, IP, WB, Functional Studiesmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue/ cell preparation (Human). (Leucocytes of a patient suffering from a LGL-type leukaemia).

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. We would recommend antibody ab8220 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 24991932
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Use under non reducing condition.

BSA is recommended for blocking.

Functional Studies Use at an assay dependent concentration. The antibody induces high-affinity conformation of LFA-1 complex.

ターゲット情報

  • 機能
    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • 関連疾患
    Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • 配列類似性
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • 翻訳後修飾
    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 95 subunit beta antibody
    • Beta 2 (antigen CD18 (p95) antibody
    • CD 18 antibody
    • CD18 antibody
    • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
    • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
    • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
    • Complement component 3 receptor 3 and 4 subunit antibody
    • Complement receptor C3 beta subunit antibody
    • Complement receptor C3 subunit beta antibody
    • Integrin beta 2 antibody
    • Integrin beta chain beta 2 antibody
    • Integrin beta-2 (ITGB2) antibody
    • Integrin beta-2 antibody
    • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
    • ITB2_HUMAN antibody
    • ITGB2 antibody
    • LAD antibody
    • LCAMB antibody
    • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
    • Leukocyte cell adhesion molecule CD18 antibody
    • LFA 1 antibody
    • LFA1 antibody
    • Lymphocyte function associated antigen 1 (LFA1) antibody
    • Lymphocyte function associated antigen 1 antibody
    • MAC 1 antibody
    • MAC1 antibody
    • Macrophage antigen 1 (mac1) beta antibody
    • MF17 antibody
    • MFI7 antibody
    • OTTHUMP00000115278 antibody
    • OTTHUMP00000115279 antibody
    • OTTHUMP00000115280 antibody
    • OTTHUMP00000115281 antibody
    • OTTHUMP00000115282 antibody
    see all

画像

  • Human peripheral blood lymphocytes stained with ab657 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22ºC. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37ºC. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4ºC. Cells were then incubated with the antibody (ab657, 1μg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

参考文献

This product has been referenced in:
  • Asadi A  et al. Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species. J Histochem Cytochem 63:646-62 (2015). Read more (PubMed: 26216140) »
  • Xiang Y  et al. Analysis on the relevance of asthma susceptibility with the alteration of integrin ß 4 expression. PLoS One 9:e95533 (2014). WB ; Human . Read more (PubMed: 24740264) »

See all 6 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell lysate - whole cell (HEK293 cells overexpressing cd18/cd11b)
Specification
HEK293 cells overexpressing cd18/cd11b
Permeabilization
No
Username

Abcam user community

Verified customer

投稿 Aug 18 2005

Thank you for your enquiry. These two antibodies were characterized using HPB-ALL or Jurkat T cells. Triplet of zones on NON-REDUCING lysates is usually observed (78-96 kDa). If you have any additional questins, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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