Customer reviews and FAQs

製品の概要

  • 製品名
    Anti-CD18 antibody [MEM-148]
    CD18 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-148] to CD18
  • 特異性
    Recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1.
  • アプリケーション
    適用あり: Flow Cyt, IP, WB, Blocking, Functional Studiesmore details
  • 種交差性
    交差種: Human
    非交差種: Pig, Monkey
  • 免疫原

    Tissue/ cell preparation (Human). (Peripheral blood mononuclear cells).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.1µg for 106 cells.

The antibody is excellent marker of activated myeloid cells.





ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non-reducing conditions.
Blocking Use at an assay dependent concentration. PubMed: 23039186
Functional Studies Use at an assay dependent concentration. Induces homotypic cell aggregation and high affinity LFA1 conformation in resting leukocytes.

ターゲット情報

  • 機能
    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • 関連疾患
    Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • 配列類似性
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • 翻訳後修飾
    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • 95 subunit beta antibody
      • Beta 2 (antigen CD18 (p95) antibody
      • CD 18 antibody
      • CD18 antibody
      • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
      • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
      • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
      • Complement component 3 receptor 3 and 4 subunit antibody
      • Complement receptor C3 beta subunit antibody
      • Complement receptor C3 subunit beta antibody
      • Integrin beta 2 antibody
      • Integrin beta chain beta 2 antibody
      • Integrin beta-2 (ITGB2) antibody
      • Integrin beta-2 antibody
      • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
      • ITB2_HUMAN antibody
      • ITGB2 antibody
      • LAD antibody
      • LCAMB antibody
      • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
      • Leukocyte cell adhesion molecule CD18 antibody
      • LFA 1 antibody
      • LFA1 antibody
      • Lymphocyte function associated antigen 1 (LFA1) antibody
      • Lymphocyte function associated antigen 1 antibody
      • MAC 1 antibody
      • MAC1 antibody
      • Macrophage antigen 1 (mac1) beta antibody
      • MF17 antibody
      • MFI7 antibody
      • OTTHUMP00000115278 antibody
      • OTTHUMP00000115279 antibody
      • OTTHUMP00000115280 antibody
      • OTTHUMP00000115281 antibody
      • OTTHUMP00000115282 antibody
      see all

    Anti-CD18 antibody [MEM-148] 画像

    • Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)
      Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)

      Human peripheral blood lymphocytes stained with ab8220 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8220, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

    • Blocking - Anti-CD18 antibody [MEM-148] (ab8220)
      Blocking - Anti-CD18 antibody [MEM-148] (ab8220)Image from Ghislin S et al., BMC Cancer. 2012 Oct 5;12:455. doi: 10.1186/1471-2407-12-455. Fig 3.; 5 October 2012, BMC Cancer 2012, 12:455
      Transmigration of A375 Human melanoma cells, blocked using ab8220.

      Melanoma cells were labeled with 10 µM of lipophilic fluorescent dye for 20 minutes at 37°C. Cells were added to wells containing either 1 µg/ml of isotypic control, or 1 µg/ml of anti-CD11a (ab3981) or anti-CD18 (ab8220) antibodies. Melanoma cells were allowed to migrate at 37°C and 5% CO2 for 48 hours. Migration was observed under a fluorescence microscope.

    Anti-CD18 antibody [MEM-148] (ab8220) 使用論文

    This product has been referenced in:
    • Ghislin S  et al. LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro. BMC Cancer 12:455 (2012). Blocking ; Human . Read more (PubMed: 23039186) »
    • Doisne JM  et al. The context of HLA-DR/CD18 complex in the plasma membrane governs HLA-DR-derived signals in activated monocytes. Mol Immunol 45:709-18 (2008). Read more (PubMed: 17719638) »

    See all 6 Publications for this product

    ab8220 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab8220 の abreview を投稿する ab8220 に関する問い合わせをする

    3 Abreviews or Q&A

    10
    Votes: Highest First

    ab8220 detects multiple bands in WB

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Dear technical service, I would appreciate information concerning the number of cells (neutrophils) and levels of antibodies that you would recommend using for the following antibodies: ab8220 primary antibody ab6785 secondary ab7002-500 secondary ...

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    There is a citation from the published methodology (Drbal et al. Blood 98(5), pp1561-1566, 2001): mAb used in paper: MEM-148 (ab8220) MEM-48 (standard anti CD18, #11-216) and other mAbs supplied...

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    Technical Services, Does CD18-ab8220 also recognize the high affinity state of Mac-1(CD11b/CD18)? Your product data sheet states that it recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high affinity ...

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    It is not clear whether ab8220 recognizes the high-affinity (activated) conformation also on Mac-1, we have only indirect indications that it is the case but no definitive unambiguous data

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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