製品の概要
- 製品名Anti-CD18 antibody [MEM-148]
CD18 一次抗体 製品一覧 - 製品の詳細Mouse monoclonal [MEM-148] to CD18
- 特異性Recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1.
- アプリケーション適用あり: Flow Cyt, IP, WB, Blocking, Functional Studiesmore details
- 種交差性交差種: Human
非交差種: Pig, Monkey - 免疫原
Tissue/ cell preparation (Human). (Peripheral blood mononuclear cells).
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- バッファーPreservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... - 精製度Protein A purified
- ポリ/モノモノクローナル
- クローン名MEM-148
- アイソタイプIgG1
- 研究分野
関連製品
- Compatible Secondaries
- Isotype control
- Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab8220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use 0.1µg for 106 cells. The antibody is excellent marker of activated myeloid cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Use under non-reducing conditions. | |
| Blocking | Use at an assay dependent concentration. PubMed: 23039186 | |
| Functional Studies | Use at an assay dependent concentration. Induces homotypic cell aggregation and high affinity LFA1 conformation in resting leukocytes. |
ターゲット情報
- 機能Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
- 関連疾患Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
- 配列類似性Belongs to the integrin beta chain family.
Contains 1 VWFA domain. - 翻訳後修飾Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
- 細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 3689 Human
- Omim: 600065 Human
- SwissProt: P05107 Human
- Unigene: 375957 Human
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別名
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
Anti-CD18 antibody [MEM-148] 画像
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![Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)](http://a.abcam.com/ps/products/8/ab8220/Images/ab8220-2-ab8220FC.jpg)
Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)Human peripheral blood lymphocytes stained with ab8220 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8220, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
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![Blocking - Anti-CD18 antibody [MEM-148] (ab8220)](http://a.abcam.com/ps/datasheet/images/8/ab8220/CD18-Primary-antibodies-ab8220-1.jpg)
Blocking - Anti-CD18 antibody [MEM-148] (ab8220)Image from Ghislin S et al., BMC Cancer. 2012 Oct 5;12:455. doi: 10.1186/1471-2407-12-455. Fig 3.; 5 October 2012, BMC Cancer 2012, 12:455Transmigration of A375 Human melanoma cells, blocked using ab8220.
Melanoma cells were labeled with 10 µM of lipophilic fluorescent dye for 20 minutes at 37°C. Cells were added to wells containing either 1 µg/ml of isotypic control, or 1 µg/ml of anti-CD11a (ab3981) or anti-CD18 (ab8220) antibodies. Melanoma cells were allowed to migrate at 37°C and 5% CO2 for 48 hours. Migration was observed under a fluorescence microscope.
Anti-CD18 antibody [MEM-148] (ab8220) 使用論文
This product has been referenced in:
- Ghislin S et al. LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro. BMC Cancer 12:455 (2012). Blocking ; Human . Read more (PubMed: 23039186) »
- Doisne JM et al. The context of HLA-DR/CD18 complex in the plasma membrane governs HLA-DR-derived signals in activated monocytes. Mol Immunol 45:709-18 (2008). Read more (PubMed: 17719638) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"