製品の概要

  • 製品名Anti-CD16+CD32 antibody [93]
    CD16+CD32 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [93] to CD16+CD32
  • 特異性ab25235 recognises CD16/Fc gamma II and CD32/Fc gamma III receptors.
  • アプリケーション適用あり: ICC/IF, Flow Cyt, Blockingmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • エピトープab25235 reacts with a conformational epitope formed by CD16 Fc gamma II and CD32 Fc gamma III receptors.
  • ポジティブ・コントロール
    • ICC/IF: RAW246.7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 3µg for 106 cells. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Blocking Use 2µg for 106 cells.

ab25235 can be used for blocking of Fc gamma receptors.

ターゲット情報

  • 関連性In mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes. The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.
  • 細胞内局在CD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
  • 参照データベース
  • 別名
    • CD16a antibody
    • CD16b antibody
    • CD32A antibody
    • CD32B antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIIa receptor antibody
    • Fc fragment of IgG low affinity IIIb receptor antibody
    • Fc gamma receptor III A antibody
    • Fc gamma receptor IIIb antibody
    • Fc gamma RIIa antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIII beta antibody
    • FcGR antibody
    • FCGR2A antibody
    • FCGR2B antibody
    • FCGR3 antibody
    • FCGR3A antibody
    • FCGR3B antibody
    • FcRIII antibody
    see all

Anti-CD16+CD32 antibody [93] 画像

  • ICC/IF image of ab25235 stained RAW246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25235, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab98420, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry analysis of BALB/c mouse splenocytes cells labeling CD16/32 with ab25235 at 1 μg/106 cells dilution. A Rat Anti-Mouse CD19-FITC was used as the secondary antibody.

Anti-CD16+CD32 antibody [93] (ab25235) 使用論文

ab25235 has not yet been referenced specifically in any publications.

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