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RabMAb

Anti-CD168 抗体 [EPR4054] (ab124729)

製品の概要

  • 製品名
    Anti-CD168 antibody [EPR4054]
    CD168 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4054] to CD168
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    corresponding to Human CD168 aa 1-100.

  • ポジティブ・コントロール
    • Lysates of: MDA-MB-435, LnCaP, T47-D, RAW264.7, PC-12. Human testis tissue
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 84 kDa.

For unpurified use at 1/1000 - 1/10000.

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

IP 1/10 - 1/100.
IHC-P 1/250.

For unpurified use at 1/50 - 1/100.

Antigen retrieval is recommended

  • 追加情報
    Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能
      Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.
    • 組織特異性
      Expressed in breast cancer cell lines and in normal breast tissue.
    • 細胞内局在
      Cell surface. Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD168 antibody
      • CD168 antigen antibody
      • HMMR antibody
      • HMMR_HUMAN antibody
      • Hyaluronan mediated motility receptor antibody
      • Hyaluronan-mediated motility receptor (RHAMM) antibody
      • IHABP antibody
      • Intracellular hyaluronic acid-binding protein antibody
      • MGC119494 antibody
      • MGC119495 antibody
      • OTTHUMP00000196920 antibody
      • Receptor for hyaluronan-mediated motility antibody
      • RHAMM antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CD168 with purified  ab124729 at 1:250 dilution (7.7 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • ab124729 (purified) at 1:100 dilution (2ug) immunoprecipitating CD168 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate.

      Lane 1 (input): MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

      Lane 2 (+): ab124729 & MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124729 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.

      Blocking and diluting buffer: 5% NFDM/TBST.



    • Predicted band size : 84 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Empty
      Lane 3: CD168 knockout HAP1 whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab124729 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab124729 was shown to specifically react with CD168 when CD168 knockout samples were used. Wild-type and CD168 knockout samples were subjected to SDS-PAGE. Ab124729 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded human testis tissue using  unpurified ab124729 at 1/50 - 1/100 dilution

    • All lanes : Anti-CD168 antibody [EPR4054] (ab124729) at 1/1000 dilution (purified)

      Lane 1 : MDA-MB-435 (Human mammary gland ductal carcinoma melanocyte) whole cell lysates at 20 µg
      Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg
      Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 84 kDa

      5% NFDM/TBST

    • All lanes : Anti-CD168 antibody [EPR4054] (ab124729)

      Lane 1 : MDA-MB-435 cell lysate
      Lane 2 : LnCaP cell lysate
      Lane 3 : T47-D cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 84 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    参考文献

    This product has been referenced in:
    • Song JM  et al. Triptolide suppresses the in vitro and in vivo growth of lung cancer cells by targeting hyaluronan-CD44/RHAMM signaling. Oncotarget 8:26927-26940 (2017). WB ; Human . Read more (PubMed: 28460475) »
    • Coulson-Thomas VJ  et al. Umbilical Cord Mesenchymal Stem Cells Suppress Host Rejection: THE ROLE OF THE GLYCOCALYX. J Biol Chem 289:23465-81 (2014). ICC/IF ; Human . Read more (PubMed: 24986866) »

    See all 2 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Loading amount
    35 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Sample
    Dog Cell lysate - whole cell (canine mammay tumor cells)
    Specification
    canine mammay tumor cells
    Treatment
    siRNA against CD168 for 24 hrs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    光芳 齋藤 

    Verified customer

    投稿 Jan 20 2015

    For ab124729 lot GR82264-8, the concentration is 1.797 mg/ml.

    The current lot we have for sale for ab108339 is lot GR106937-4 and that is 0.193 mg/ml.

    Thank you for contacting Abcam earlier today.

    Please find attached the IHC-P protocol for both ab108339 & ab124729.

    If there is anything else I can help you with, please let me know.

    Thank you for your email.



    I am glad to hear that the free of charge replacement worked as expected and you have obtained satisfactory results.



    If you wish, you could submit your results as feedback using our Ab...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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