製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CD166
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Cow, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human CD166.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human normal skin FFPE section.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
  • 組織特異性
    Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.
  • 配列類似性
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • ドメイン
    The CD6 binding site is located in the N-terminal Ig-like domain.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activated leukocyte cell adhesion molecule antibody
    • ALCAM antibody
    • ALCAM protein antibody
    • CD 166 antibody
    • CD166 antibody
    • CD166 antigen antibody
    • CD166_HUMAN antibody
    • FLJ3851 antibody
    • FLJ38514 antibody
    • MEMD antibody
    • MGC71733 antibody
    see all

画像

  • IHC image of ab78649 staining in human normal skin FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78649, 1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

プロトコール

参考文献

This product has been referenced in:
  • Landa-Solís C  et al. Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications. Cell Tissue Bank : (2015). IF . Read more (PubMed: 26220398) »

See 1 Publication for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tooth)
Antigen retrieval step
None
Permeabilization
No
Specification
tooth
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 02 2015



I am pleased to hear you would like to accept our offer and test ab78649 in sheep. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forsheep and include this code in ...

Read More

Thank you very much for your interest in ab78649. I am not exactly sure, how we could help in this case. To our knowledge, ab78649 has not been tested insheep. Therefore, if you would like to test this, I can offer a discount off a future purchase if y...

Read More

Comme convenu je vous ai fait parvenir un devis pourune unité d'anti-CD34 (FITC) http://www.abcam.com/ab78165 et une unité d'anti-CD166 http://www.abcam.com/ab78649.

Je me permets de vous rappeller que nous offrons jusqu'au 2 m...

Read More



Nous avons à notre catalogue les anti-CD34 suivants qui, je pense, peuvent vous intéresser :

anti-CD34 (FITC) : http://www.abcam.com/ab18227, http://www.abcam.com/ab46924, http://www.abcam.com/ab78165
anti-CD34 (A...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録