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RabMAb

Anti-CD163 抗体 [SP96] - N-terminal (ab100909)

製品の概要

  • 製品名
    Anti-CD163 antibody [SP96] - N-terminal
    CD163 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP96] to CD163 - N-terminal
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Human CD163 (N terminal).
    Database link: Q86VB7

  • ポジティブ・コントロール
    • IHC-P: Human lung, bone marrow, reactive lymph node, thymus, tonsil, ovary and esophagus tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.

Boil tissue section in EDTA pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Incubate with primary antibody for 30 minutes at room temperature.

ターゲット情報

  • 機能
    Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
    After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
  • 組織特異性
    Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
  • 配列類似性
    Contains 9 SRCR domains.
  • ドメイン
    The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
  • 翻訳後修飾
    A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
    Phosphorylated.
  • 細胞内局在
    Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C163A_HUMAN antibody
    • CD 163 antibody
    • CD163 antibody
    • CD163 antigen antibody
    • CD163 molecule antibody
    • Hemoglobin scavenger receptor antibody
    • M130 antibody
    • M130 antigen precursor antibody
    • Macrophage associated antigen antibody
    • MM130 antibody
    • OTTHUMP00000238617 antibody
    • OTTHUMP00000238618 antibody
    • OTTHUMP00000238619 antibody
    • OTTHUMP00000238620 antibody
    • SCARI1 antibody
    • Scavenger receptor cysteine rich type 1 protein M130 antibody
    • sCD163 antibody
    • Soluble CD163 antibody
    see all

画像

  • Formalin-fixed, paraffin-embedded human lung tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bone marrow tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human ovary tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human esophagus tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

プロトコール

参考文献

ab100909 has not yet been referenced specifically in any publications.

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