Anti-CD163 抗体 [10D6], prediluted (ab74604)
Key features and details
- Mouse monoclonal [10D6] to CD163, prediluted
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CD163 antibody [10D6], prediluted
CD163 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [10D6] to CD163, prediluted -
由来種
Mouse -
特異性
ab74604 may be useful in the localization of monocytes and most macrophage types as well as aiding further study into the function and expression of CD163 antigen itself. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant protein encoding of domains 1-4 of the N-terminal region of human CD163.
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エピトープ
Domains 1 to 4 of the N-terminal region -
ポジティブ・コントロール
- Human placenta tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
10D6 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74604の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Ready to use. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Ready to use. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. -
組織特異性
Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. -
配列類似性
Contains 9 SRCR domains. -
ドメイン
The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes. -
翻訳後修飾
A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Phosphorylated. -
細胞内局在
Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells. - Information by UniProt
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参照データベース
- Entrez Gene: 9332 Human
- Omim: 605545 Human
- SwissProt: Q86VB7 Human
- Unigene: 504641 Human
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別名
- C163A_HUMAN antibody
- CD 163 antibody
- CD163 antibody
see all
画像
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ab74604 staining CD163 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 4 minutes at 36°C; antigen retrieval was by heat mediation in EDTA pH 8.0. Samples were incubated with the prediluted primary antibody for 20 minutes at 37°C. An HRP polymer-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
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ab74604 staining CD163 in human placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note membrane staining of monocytes and macrophages
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab74604 は 22 報の論文で使用されています。
- Obradovic A et al. Single-cell protein activity analysis identifies recurrence-associated renal tumor macrophages. Cell 184:2988-3005.e16 (2021). PubMed: 34019793
- Karadimou G et al. TLR7 Expression Is Associated with M2 Macrophage Subset in Calcific Aortic Valve Stenosis. Cells 9:N/A (2020). PubMed: 32708790
- Marques P et al. The role of the tumour microenvironment in the angiogenesis of pituitary tumours. Endocrine 70:593-606 (2020). PubMed: 32946040
- Segovia C et al. Inhibition of a G9a/DNMT network triggers immune-mediated bladder cancer regression. Nat Med 25:1073-1081 (2019). PubMed: 31270502
- Shiba M et al. Presence of increased inflammatory infiltrates accompanied by activated dendritic cells in the left atrium in rheumatic heart disease. PLoS One 13:e0203756 (2018). PubMed: 30261069