Anti-CD162 抗体 [HECA-452] (ab110096)

製品の概要

  • 製品名Anti-CD162 antibody [HECA-452]
    CD162 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [HECA-452] to CD162
  • アプリケーション適用あり: IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Raised against a stromal preparation of human tonsils

  • ポジティブ・コントロール
    • Human spleen or tonsil tissue. Human peripheral blood lymphocytes.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab110096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab35768-Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
    (Microbial infection) Acts as a receptor for enterovirus 71.
  • 組織特異性Expressed on neutrophils, monocytes and most lymphocytes.
  • 翻訳後修飾Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
    Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 162 antibody
    • CD162 antibody
    • CD162 antigen antibody
    • CLA antibody
    • Cutaneous lymphocyte associated associated antigen antibody
    • P selectin glycoprotein ligand 1 antibody
    • P selectin glycoprotein ligand 1 precursor antibody
    • P-selectin glycoprotein ligand 1 antibody
    • PSGL 1 antibody
    • PSGL-1 antibody
    • PSGL1 antibody
    • Selectin P ligand antibody
    • SELPL_HUMAN antibody
    • SELPLG antibody
    see all

Anti-CD162 antibody [HECA-452] 画像

  • ab110096 at 10µg/ml staining CD162 in Formalin/PFA-fixed paraffin-embedded Human spleen tissue.
  • ab110096 at 10µg/ml staining CD162 in Formalin/PFA-fixed paraffin-embedded Human tonsil tissue.

Anti-CD162 antibody [HECA-452] (ab110096) 使用論文

ab110096 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (LPS-inflamed lung)
Permeabilization No
Specification LPS-inflamed lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
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投稿 Mar 07 2016

I am very pleased to hear you would like to accept our offer and test ab110096 in ICC/IF. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forICC/IF and include this code in the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"